Anticorps Polyclonal de lapin anti-LY6D

• Phare
• Validé par KD/KO

LY6D Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(11)

Avis (1)

N° de cat : 17361-1-AP

Synonymes

E48, E48 antigen, Ly 6D, Ly-6D, Lymphocyte antigen 6D

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Galerie de données de validation

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  WB analysis of A431 using 17361-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17361-1-AP (LY6D antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 17361-1-AP

  WB result of LY6D antibody (17361-1-AP; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-LY6D transfected A431 cells.

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  IHC staining of human tonsillitis using 17361-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17361-1-AP (LY6D antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 17361-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17361-1-AP (LY6D Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 17361-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17361-1-AP (LY6D Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin cancer using 17361-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17361-1-AP (LY6D antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin cancer using 17361-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17361-1-AP (LY6D antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A431,
• Résultats positifs en IHC: tissu de cancer de la peau humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 6 publications below

Informations sur le produit

17361-1-AP cible LY6D dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LY6D Protéine recombinante Ag10714
• Nom complet: lymphocyte antigen 6 complex, locus D
• Masse moléculaire calculée: 128 aa, 13 kDa
• Poids moléculaire observé: 13-14 kDa
• Numéro d’acquisition GenBank: BC031330
• Symbole du gène: LY6D
• Identification du gène (NCBI): 8581
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LY6D, also named as E48 and ThB, may act as a specification marker at earliest stage specification of lymphocytes between B- and T-cell development. It marks the earliest stage of B-cell specification.

Publications

Species	Application	Title
mouse,human	IF	Nat Commun; AP-1 and TGFß cooperativity drives non-canonical Hedgehog signaling in resistant basal cell carcinoma.; Authors - Catherine D Yao
mouse	IHC,IF	Cell Rep; Autocrine activation of MAPK signaling mediates intrinsic tolerance to androgen deprivation in LY6D prostate cancer cells; Authors - Ivana Steiner
mouse	WB	Diabetes; PMID: 28325853; Authors - Yu-Yao Zhang
mouse	IF	Elife; RUNX1 marks a luminal castration-resistant lineage established at the onset of prostate development.; Authors - Renaud Mevel
mouse,human	IHC,IF	Cancers (Basel); Lung Adenocarcinoma Mouse Models Based on Orthotopic Transplantation of Syngeneic Tumor-Initiating Cells Expressing EpCAM, SCA-1, and Ly6d.; Authors - Takashi Semba ; KD Validated
human	WB	PLoS One; Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer.; Authors - Kui Xiao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

James (Verified Customer) (05-23-2025)

This antibody did not work for my samples (liver protein extract). It gave unspecific bands over 130 kDa

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: liver cells

LY6D Antibody Western Blot validation (1/1000 dilution) in liver cells (Cat no:17361-1-AP)

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