Anticorps Polyclonal de lapin anti-LUZP1

• Phare
• Validé par KD/KO

LUZP1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 17483-1-AP

Synonymes

KIAA0601, leucine zipper protein 1, LUZP, LUZP1

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Galerie de données de validation

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  WB analysis using 17483-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17483-1-AP (LUZP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17483-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17483-1-AP (LUZP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17483-1-AP

  IP result of anti-LUZP1 (IP:17483-1-AP, 4ug; Detection:17483-1-AP 1:10000) with mouse brain tissue lysate 1680 ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

17483-1-AP cible LUZP1 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LUZP1 Protéine recombinante Ag11518
• Nom complet: leucine zipper protein 1
• Masse moléculaire calculée: 1026 aa, 115 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC051733
• Symbole du gène: LUZP1
• Identification du gène (NCBI): 7798
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LUZP1, also known as LUZP, is predominantly expressed in the brain, whereas it is present in the nucleus of neurons. LUZP1 is a centrosome, actin, and midbody-localizing protein implicated in ciliogenesis regulation and actin cytoskeleton stability (PMID: 33869174). LUZP1 null mice present defective neural tube closure and cardiovascular problems, which cause perinatal death. In addition, LUZP1 has also been implicated in the etiology of diseases like the 1p36 and the Townes-Brocks syndromes (PMID: 35738212).

Publications

Species	Application	Title
human	WB	Elife; LUZP1, a novel regulator of primary cilia and the actin cytoskeleton, is a contributing factor in Townes-Brocks Syndrome.; Authors - Laura Bozal-Basterra
human	WB	Front Oncol; The Circ_0001367/miR-545-3p/LUZP1 Axis Regulates Cell Proliferation, Migration and Invasion in Glioma Cells.; Authors - Xuchen Dong
mouse	WB	J Biol Chem; Activating transcription factor 4 (ATF4) promotes skeletal muscle atrophy by forming a heterodimer with the transcriptional regulator C/EBPβ.; Authors - Scott M Ebert
		Eur J Cell Biol; LUZP1: A new player in the actin-microtubule cross-talk.; Authors - João Gonçalves
mouse	WB	Biochem Cell Biol; Chromatin remodeling factor CECR2 forms tissue-specific complexes with CCAR2 and LUZP1.; Authors - Farshad Niri
human,mouse	WB,IF,IP	Biochemistry; Identification of Filamin A Mechanobinding Partner II: Fimbacin Is a Novel Actin Cross-Linking and Filamin A Binding Protein; Authors - Jiale Wang