Anticorps Polyclonal de lapin anti-LRDD

LRDD Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 12119-1-AP

Synonymes

LRDD, PIDD, PIDD1

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Galerie de données de validation

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  WB analysis of L02 using 12119-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12119-1-AP (LRDD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12119-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12119-1-AP (LRDD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 12119-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12119-1-AP (LRDD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 12119-1-AP

  IP result of anti-LRDD (IP:12119-1-AP, 4ug; Detection:12119-1-AP 1:500) with L02 cells lysate 2800ug.

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  IHC staining of human pancreas using 12119-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 12119-1-AP (LRDD antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 12119-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 12119-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12119-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12119-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12119-1-AP (LRDD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 12119-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 12119-1-AP (LRDD antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 12119-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12119-1-AP (LRDD antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 12119-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12119-1-AP (LRDD antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 12119-1-AP

  Immunofluorescent analysis of Hela cells, using LRDD antibody 12119-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HEK-293 using 12119-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using LRDD antibody (12119-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules L02, cellules HEK-293
• Résultats positifs en IP: cellules L02
• Résultats positifs en IHC: tissu pulmonaire humain, tissu de cancer du poumon humain, tissu hépatique humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

12119-1-AP cible LRDD dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LRDD Protéine recombinante Ag2761
• Nom complet: leucine-rich repeats and death domain containing
• Masse moléculaire calculée: 893 aa, 98 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC014904
• Symbole du gène: LRDD
• Identification du gène (NCBI): 55367
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

P53-induced protein with a death domain (PIDD/LRDD) is a component of the PIDDosome. PIDD contains 910 residues with 7 leucine rich repeats (LRRs), 2 ZU-5 domains and a C-terminal death domain (DD). PIDD can be cleaved into shorter fragments generating a PIDD-N fragment of 48 kD (residues 1-446), a PIDD-C fragment of 51 kD (residues 447-910) and a PIDD-CC fragment of 37 kD (residues 589-910). Auto-cleavage of PIDD determines the downstream signaling events. The PIDD-C fragment mediates activation of NFκB via the recruitment of RIP1 and NEMO, while PIDD-CC causes caspase-2 activation, which leads to apoptosis.

Publications

Species	Application	Title
mouse	WB	Neural Regen Res; Neat1 decreases neuronal apoptosis after oxygen and glucose deprivation.; Authors - Wei-Na Chai
mouse	WB	Exp Neurol; Microglia aggravate white matter injury via C3/C3aR pathway after experimental subarachnoid hemorrhage; Authors - Lei Yang