Anticorps Polyclonal de lapin anti-LRAT

LRAT Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 12815-1-AP

Synonymes

LRAT



Applications testées

Résultats positifs en WBtissu testiculaire de souris, cellules Y79

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

WBSee 3 publications below

Informations sur le produit

12815-1-AP cible LRAT dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène LRAT Protéine recombinante Ag3556
Nom complet lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase)
Masse moléculaire calculée 230 aa, 26 kDa
Poids moléculaire observé 26 kDa, 50-54 kDa
Numéro d’acquisition GenBankBC031053
Symbole du gène LRAT
Identification du gène (NCBI) 9227
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LRAT belongs to the H-rev107 family. LRAT transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, LRAT monomers (26kDa) interact in membranes and form functional homodimers (50-54 kDa) through protein-protein interactions and disulfide bond formation.Defects in LRAT are a cause of severe early-onset retinal dystrophy (RD). This antibody was generated against the internal residues of human LRAT. It is predicted to detect endogenous LRAT.

Protocole

Product Specific Protocols
WB protocol for LRAT antibody 12815-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

iScience

Control by the brain of vitamin A homeostasis

Authors - Peter I Imoesi
ratWB

Am J Pathol

Impaired Rhodopsin Generation in the Rat Model of Diabetic Retinopathy.

Authors - Volha V Malechka
mouseWB

J Food Biochem

Inhibitory effect of chitooligosaccharides on retinol metabolism and bioavailability in mice.

Authors - Wei Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Peter (Verified Customer) (12-03-2018)

The Proteintech LRAT primary antibody works so well and the antibody is prepared using BSA it can be use repeatedly when stored at -20. The strength of the antibody remains the same over a period of 6 months or more.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Rat Liver Tissue
LRAT Antibody Western Blot, validation (1:500 dilution) in Rat Liver Tissue (Cat no:12815-1-AP)