Anticorps Polyclonal de lapin anti-LPL

LPL Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 16899-1-AP

Synonymes

HDLCQ11, LIPD, lipoprotein lipase, LPL

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Galerie de données de validation

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  WB analysis of MCF-7 using 16899-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16899-1-AP (LPL Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 16899-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16899-1-AP (LPL Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16899-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16899-1-AP (LPL Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HeLa, tissu de muscle squelettique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

16899-1-AP cible LPL dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LPL Protéine recombinante Ag10431
• Nom complet: lipoprotein lipase
• Masse moléculaire calculée: 475 aa, 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC011353
• Symbole du gène: LPL
• Identification du gène (NCBI): 4023
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LPL(lipoprotein lipase) is also named as LIPD and belongs to the AB hydrolase superfamily. LPL catalyses the hydrolysis of the triacylglycerol component of circulating chylomicrons and very low density lipoproteins. It plays a role in the binding of lipoprotein particles to cell-surface molecules, including sulfated glycosaminoglycans (GAGs)(PMID:21177248). The gene encodes a protein of 475 amino acids that becomes a mature protein of 448 residues after cleavage of a signal peptide. This protein is highly glycosylated and functions as a head-to-tail dimer under well-defined conditions(PMID:11154699).

Publications

Species	Application	Title
mouse	WB	Diabetes; Rab8a Deficiency in Skeletal Muscle Causes Hyperlipidemia and Hepatosteatosis Via Impairment of Muscle Lipid Uptake and Storage.; Authors - Qiaoli Chen
		Diabetes; Tissue-specific splicing and dietary interaction of a mutant As160 allele determine muscle metabolic fitness in rodents.; Authors - Xinyu Yang