Anticorps Polyclonal de lapin anti-LPCAT1

• Phare
• Validé par KD/KO

LPCAT1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

32

N° de cat : 16112-1-AP

Synonymes

Acyltransferase like 2, AYTL2, LPC acyltransferase 1, lpcat, LPCAT 1, LPCAT1, Lyso PAF acetyltransferase, LysoPAFAT, LysoPC acyltransferase 1, PFAAP3

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Galerie de données de validation

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  WB analysis using 16112-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16112-1-AP (LPCAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16112-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16112-1-AP (LPCAT1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16112-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16112-1-AP (LPCAT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 16112-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 16112-1-AP (LPCAT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 16112-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16112-1-AP (LPCAT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 16112-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 16112-1-AP (LPCAT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 16112-1-AP

  IP result of anti-LPCAT1 (IP:16112-1-AP, 4ug; Detection:16112-1-AP 1:4000) with mouse brain tissue lysate 1280 ug.

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  IP experiment of mouse brain using 16112-1-AP

  IP result of anti-LPCAT1 (IP:16112-1-AP, 3ug; Detection:16112-1-AP 1:4000) with mouse brain tissue lysate 3600ug.

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  IHC staining of human breast cancer using 16112-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16112-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16112-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 16112-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 16112-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 16112-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16112-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 16112-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 16112-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 16112-1-AP (LPCAT1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, tissu cérébral de rat, tissu cérébral de souris, tissu pulmonaire de rat, tissu pulmonaire de souris, tissu pulmonaire humain, tissu splénique de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain, tissu pulmonaire de souris, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 22 publications below
• IHC: See 15 publications below
• IF: See 8 publications below

Informations sur le produit

16112-1-AP cible LPCAT1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LPCAT1 Protéine recombinante Ag9035
• Nom complet: lysophosphatidylcholine acyltransferase 1
• Masse moléculaire calculée: 534 aa, 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC020166
• Symbole du gène: LPCAT1
• Identification du gène (NCBI): 79888
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LPCAT1, also named as AYTL2, PFAAP3 and LysoPAFAT, belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. It is a key enzyme for remodeling phospholipids, including phosphatidylcholine. The expression level of LPCAT1 is able to differentiate prostate cancer from noncancerous prostatic changes, and correlates to the tumor grade of prostate cancer. LPCAT1 possesses both acyltransferase and acetyltransferase activities. It mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC).

Publications

Species	Application	Title
human	WB,IHC	Cell Metab; Oncogene Amplification in Growth Factor Signaling Pathways Renders Cancers Dependent on Membrane Lipid Remodeling.; Authors - Junfeng Bi ; KD Validated
human	WB	J Hepatol; Lysophosphatidylcholine acyltransferase 1 altered phospholipid composition and regulated hepatoma progression.; Authors - Morita Yoshifumi Y ; KD Validated
HUMAN	WB	Curr Biol; Neutral lipid stores and lipase PNPLA5 contribute to autophagosome biogenesis.; Authors - Nicolas Dupont
human	WB,IF	Proc Natl Acad Sci U S A; Regulation of stem cell function and neuronal differentiation by HERV-K via mTOR pathway.; Authors - Tongguang Wang ; KD Validated
human	IHC	Elife; Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids.; Authors - Marko Z Nikolić
human	WB	Front Immunol; Identification of molecular subtypes based on PANoptosis-related genes and construction of a signature for predicting the prognosis and response to immunotherapy response in hepatocellular carcinoma; Authors - Jinfeng Zhu ; KD Validated