Anticorps Polyclonal de lapin anti-LONP1

• Phare
• Validé par KD/KO

LONP1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

85

Avis (1)

N° de cat : 15440-1-AP

Synonymes

hLON, LON, lon peptidase 1, mitochondrial, Lon protease like protein, LonHS, LONP, LONP1, PIM1, PRSS15, Serine protease 15

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Galerie de données de validation

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  WB analysis of Jurkat cells using 15440-1-AP

  WB result of LONP1 antibody (15440-1-AP, 1:6000) with si-Control and si-LONP1 transfected Jurkat cells.

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  WB analysis using 15440-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15440-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 15440-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  WB analysis of rat lung using 15440-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of human heart using 15440-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15440-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15440-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15440-1-AP (LONP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 15440-1-AP

  IP result of anti-LONP1 (IP:15440-1-AP, 3ug; Detection:15440-1-AP 1:1000) with mouse heart tissue lysate 9500ug.

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  IHC staining of human lung cancer using 15440-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15440-1-AP (LONP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15440-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15440-1-AP (LONP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15440-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15440-1-AP (LONP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15440-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15440-1-AP (LONP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15440-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 15440-1-AP (LONP1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 15440-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LONP1 antibody (15440-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 15440-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LONP1 antibody (15440-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 15440-1-AP

  Immunofluorescent analysis of HepG2 cells using 15440-1-AP (LONP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, tissu cardiaque de rat, tissu cardiaque de souris, tissu cardiaque humain, tissu cérébral de rat, tissu cérébral de souris, tissu pulmonaire de rat
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 76 publications below
• IHC: See 3 publications below
• IF: See 10 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

15440-1-AP cible LONP1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poulet, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LONP1 Protéine recombinante Ag7196
• Nom complet: lon peptidase 1, mitochondrial
• Masse moléculaire calculée: 106 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC000235
• Symbole du gène: LONP1
• Identification du gène (NCBI): 9361
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then progressively degraded(PMID:15870080). LONP1 has some isoforms with the MW of 106 kDa, 100 kDa and 86 kDa.

Publications

Species	Application	Title
human	WB	Nature; A cytosolic surveillance mechanism activates the mitochondrial UPR; Authors - F X Reymond Sutandy ; KD Validated
human	WB	Cell Metab; A High-Density Human Mitochondrial Proximity Interaction Network.; Authors - Hana Antonicka
monkey	WB,IF	Nat Cell Biol; MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.; Authors - Tim König
human	WB	Mol Cell; Circadian Oscillation of Sulfiredoxin in the Mitochondria.; Authors - In Sup Kil ; KD Validated
mouse	WB	Sci Adv; Fidelity of translation initiation is required for coordinated respiratory complex assembly.; Authors - Danielle L Rudler
mouse	WB,IHC	Sci Adv; Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4.; Authors - Qiqi Guo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

B (Verified Customer) (01-28-2023)

It is okay to detect endogenous LONP1 although there are weak non specific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse cortex lysate

LONP1 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:15440-1-AP)