Anticorps Polyclonal de lapin anti-ERGIC-53

• Phare
• Validé par KD/KO

ERGIC-53 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

23

Avis (2)

N° de cat : 13364-1-AP

Synonymes

ERGIC 53, ERGIC-53, F5F8D, FMFD1, gp58, Lectin mannose binding 1, lectin, mannose binding, 1, LMAN1, MCFD1, MR60, Protein ERGIC 53

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Galerie de données de validation

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  WB analysis using 13364-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13364-1-AP (ERGIC-53 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13364-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13364-1-AP (ERGIC-53 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13364-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13364-1-AP (ERGIC-53 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 13364-1-AP

  IP result of anti-ERGIC-53 (IP:13364-1-AP, 4ug; Detection:13364-1-AP 1:400) with HepG2 cells lysate 1000 ug.

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  IHC staining of human stomach cancer using 13364-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13364-1-AP (ERGIC-53 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 13364-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13364-1-AP (ERGIC-53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13364-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13364-1-AP (ERGIC-53 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13364-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13364-1-AP (ERGIC-53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 13364-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ERGIC-53 antibody (13364-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of A549 using 13364-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 13364-1-AP (ERGIC-53 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules MCF-7, tissu cardiaque de rat, tissu cardiaque de souris, tissu cérébral de souris, tissu cérébral humain, tissu splénique de rat, tissu splénique de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 13 publications below

Informations sur le produit

13364-1-AP cible ERGIC-53 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ERGIC-53 Protéine recombinante Ag4183
• Nom complet: lectin, mannose-binding, 1
• Masse moléculaire calculée: 510 aa, 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC032330
• Symbole du gène: ERGIC-53
• Identification du gène (NCBI): 3998
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ERGIC-53 (also known as LMAN1 or MR60) is a membrane mannose-specific lectin that selectively transports its cargo proteins from ER to ER-Golgi intermediate compartment (ERGIC) and Golgi, functioning as a cargo transport receptor for glycoproteins (PMID: 24664723; 10559958). Mutations in ERGIC-53 cause combined deficiency of coagulation factors V and VIII (PMID: 9546392).

Publications

Species	Application	Title
human	WB	Cell Metab; TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.; Authors - Dong Huang
human	WB	Cell Metab; Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.; Authors - Xiao Wang
mouse	WB	Nat Cell Biol; Manganese regulation of COPII condensation controls circulating lipid homeostasis; Authors - Xiao Wang
human	IF	Nat Commun; ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling; Authors - Maximilian Hirschenberger
mouse	IF	Nat Commun; Homeostatic regulation of STING by retrograde membrane traffic to the ER.; Authors - Kojiro Mukai
human	WB	Nat Commun; Improved secretion of glycoproteins using an N-glycan-restricted passport sequence tag recognized by cargo receptor.; Authors - Hirokazu Yagi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

pitna (Verified Customer) (05-26-2021)

for Ergic Immunoprecipitation validation for human brain

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: human brain

ERGIC-53 Antibody Western Blot,Immunoprecipitation validation (1:500 dilution) in human brain (Cat no:13364-1-AP)

FH

Boyan (Verified Customer) (02-19-2020)

For WB, this antibody could detect endogenous Ergic53 with a very clean band.

• Applications: Western Blot,