Anticorps Polyclonal de lapin anti-LIPA

• Phare
• Validé par KD/KO

LIPA Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA, IF

Conjugaison

Non conjugué

Publications

8

N° de cat : 12956-1-AP

Synonymes

LipaseA, Lipase A, EC:3.1.1.13, Cholesteryl esterase, CESD

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Galerie de données de validation

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  WB analysis of HEK-293 using 12956-1-AP

  WB result of LIPA antibody (12956-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LIPA transfected HEK-293 cells.

• 

  WB analysis of HT-1080 using 12956-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 12956-1-AP (LIPA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 12956-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 12956-1-AP (LIPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 12956-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12956-1-AP (LIPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12956-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12956-1-AP (LIPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 12956-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 12956-1-AP (LIPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12956-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12956-1-AP (LIPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12956-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12956-1-AP (LIPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HT-1080 using 12956-1-AP

  IP result of anti-LIPA (IP:12956-1-AP, 4ug; Detection:12956-1-AP 1:2000) with HT-1080 cells lysate 3600 ug.

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  IHC staining of human colon cancer using 12956-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12956-1-AP (LIPA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12956-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12956-1-AP (LIPA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HT-1080, cellules HEK-293, cellules HeLa, cellules HepG2, cellules NIH/3T3, cellules RAW 264.7
• Résultats positifs en IP: cellules HT-1080,
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

12956-1-AP cible LIPA dans les applications de WB, IHC, IP, ELISA, IF et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LIPA Protéine recombinante Ag3627
• Nom complet: lipase A, lysosomal acid, cholesterol esterase
• Masse moléculaire calculée: 399 aa, 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC012287
• Symbole du gène: LIPA
• Identification du gène (NCBI): 3988
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human acid lipase/cholesteryl esterase (LIPA) is a 46-kDa glycoprotein required for the lysosomal hydrolysis of cholesteryl esters and triglycerides that cells acquire through the receptor-mediated endocytosis of low-density lipoproteins(PMID:8432549). Defects in LIPA are the cause of Wolman disease (WOD)(PMID:8146180) and cholesteryl ester storage disease (CESD)(PMID:9633819). It has 2 isoforms produced by alternative splicing with the molecular weight of 45 kDa and 39 kDa. The full length protein has a signal peptide and several glycosylation sites.

Publications

Species	Application	Title
human	WB	Cancer Commun (Lond); Squalene epoxidase promotes colorectal cancer cell proliferation through accumulating calcitriol and activating CYP24A1-mediated MAPK signaling.; Authors - Luwei He
rat	WB	Phytomedicine; Morroniside, a novel GATA3 binding molecule, inhibits hepatic stellate cells activation by enhancing lysosomal acid lipase expression.; Authors - Lin An
rat	WB	J Oncol; CircRNA-Associated ceRNA Network Reveals Focal Adhesion and Metabolism Pathways in Neuropathic Pain; Authors - Kun Wang
human,mouse	WB,IHC,IF,IP	Cell Death Dis; Molecular and histological traits of reduced lysosomal acid lipase activity in the fatty liver.; Authors - Simone Carotti
		Hepatol Commun; Evidence for a Novel Regulatory Interaction Involving Cyclin D1, Lipid Droplets, Lipolysis, and Cell Cycle Progression in Hepatocytes.; Authors - Heng Wu
mouse	WB	Nutrients; Moderate Treadmill Exercise Alleviates NAFLD by Regulating the Biogenesis and Autophagy of Lipid Droplet; Authors - Yangjun Yang