Anticorps Polyclonal de lapin anti-LIMK2

LIMK2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 12350-1-AP

Synonymes

LIM domain kinase 2, LIMK 2, LIMK2

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 12350-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12350-1-AP (LIMK2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Informations sur le produit

12350-1-AP cible LIMK2 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LIMK2 Protéine recombinante Ag3011
• Nom complet: LIM domain kinase 2
• Masse moléculaire calculée: 686 aa, 78 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC013051
• Symbole du gène: LIMK2
• Identification du gène (NCBI): 3985
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The family of LIM kinase (LIMK) includes two similar proteins, LIMK1 and LIMK2, that share overall identity of 53% with 75%, 55%, and 42% amino acid identity in the LIM, kinase, and PDZ domains, respectively. LIMK2 regulates actin cytoskeletal reorganization through phosphorylating and inactivating cofilin, an actin-depolymerizing factor of actin filaments. It also may associate with gamma-tubulin and play a role in mitotic spindle assembly. High levels of LIMK2 are evident in uterus, thymus, and spleen whereas lower levels are observed in brain, heart, liver, stomach, skeletal muscle, and testes according to the results of western blotting(PMID:16399995). It has 3 isoforms produced by alternative splicing of the molecular weight of 70-78 kDa. It can be detected a band of 47 kDa or 50-55 kDa in the testes because the antibody is recognizing the testis-specific variant of LIMK2 and the this difference could be due to species variation(PMID:17928627).

Publications

Species	Application	Title
human	WB	Adv Healthc Mater; Modification of PLGA Scaffold by MSC-Derived Extracellular Matrix Combats Macrophage Inflammation to Initiate Bone Regeneration via TGF-β-Induced Protein.; Authors - Moyuan Deng
human	IF	J Cachexia Sarcopenia Muscle; RhoA/ROCK2 signalling is enhanced by PDGF-AA in fibro-adipogenic progenitor cells: implications for Duchenne muscular dystrophy.; Authors - Esther Fernández-Simón
human	IHC	Theranostics; NUDT21 inhibits bladder cancer progression through ANXA2 and LIMK2 by alternative polyadenylation.; Authors - Ming Xiong
human	WB,IHC	Cell Death Dis; Imbalanced LIMK1 and LIMK2 expression leads to human colorectal cancer progression and metastasis via promoting β-catenin nuclear translocation.; Authors - Yue Zhang
mouse	WB	PLoS One; ECM-dependent HIF induction directs trophoblast stem cell fate via LIMK1-mediated cytoskeletal rearrangement.; Authors - Choi Hwa J HJ
human	WB,IF	Mol Biotechnol; Stratifin (SFN) Regulates Cervical Cancer Cell Proliferation, Apoptosis, and Cytoskeletal Remodeling and Metastasis Progression Through LIMK2/Cofilin Signaling; Authors - Naiyi Du