Anticorps Polyclonal de lapin anti-EPLIN

• Phare
• Validé par KD/KO

EPLIN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 16639-1-AP

Synonymes

LIMA1, LIM domain and actin binding 1, LIM domain and actin-binding protein 1, PP624, SREBP3

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Galerie de données de validation

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  WB analysis of HEK-293 using 16639-1-AP

  WB result of EPLIN antibody (16639-1-AP, 1:1000) with si-Control and si-EPLIN transfected HEK293 cells.

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  WB analysis of HUVEC using 16639-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 16639-1-AP (EPLIN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 16639-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16639-1-AP (EPLIN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 16639-1-AP

  IP result of anti-EPLIN (IP:16639-1-AP, 4ug; Detection:16639-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IP experiment of HepG2 using 16639-1-AP

  IP result of anti-EPLIN (IP:16639-1-AP, 4ug; Detection:16639-1-AP 1:8000) with HepG2 cells lysate 1480 ug.

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  IHC staining of human prostate cancer using 16639-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16639-1-AP (EPLIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 16639-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16639-1-AP (EPLIN antibody at dilution of 1:50 (under 10x lens).

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  IF Staining of PC-3 using 16639-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using EPLIN antibody (16639-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 16639-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EPLIN antibody (16639-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 16639-1-AP

  Immunofluorescent analysis of HepG2 cells, using LIMA1 antibody 16639-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules HEK-293, tissu placentaire humain
• Résultats positifs en IP: cellules HepG2, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules PC-3, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

16639-1-AP cible EPLIN dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EPLIN Protéine recombinante Ag9994
• Nom complet: LIM domain and actin binding 1
• Masse moléculaire calculée: 85 kDa
• Poids moléculaire observé: 90 kDa, 110 kDa
• Numéro d’acquisition GenBank: BC001247
• Symbole du gène: EPLIN
• Identification du gène (NCBI): 51474
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. The 110 kDa EPLIN-β isoform represents the full-length protein and the 90 kDa EPLIN-α isoform lacks the amino-terminal 160 amino acids. EPLIN-a expression is often down-regulated in cancerous cells and tissues. (PMID: 10806352, 31644899)

Publications

Species	Application	Title
human	WB	Exp Mol Med; The deubiquitinating enzyme STAMBP is a newly discovered driver of triple-negative breast cancer progression that maintains RAI14 protein stability; Authors - Qianqian Yang
human	IHC	J Cell Biol; Rab40-Cullin5 complex regulates EPLIN and actin cytoskeleton dynamics during cell migration.; Authors - Erik S Linklater
mouse	IF	Acta Histochem Cytochem; EPLINβ Is Involved in the Assembly of Cadherin-catenin Complexes in Osteoblasts and Affects Bone Formation.; Authors - Shihoko Miyazaki
human	WB	Proteomics; A proteomic signature and potential pharmacological opportunities in the adaptive resistance to MEK and PI3K kinase inhibition in pancreatic cancer cells; Authors - Alain Aguilar-Valdés
human	WB	Exp Cell Res; Nuclear-cytoplasmic translocation of SQSTM1/p62 protein enhances ESCC cell migration and invasion by stabilizing EPLIN expression; Authors - Zou Liu ; KD Validated
human	IHC,IF	Cell Biol Toxicol; M6A modification-mediated LIMA1 promotes the progression of hepatocellular carcinoma through the wnt-βcatenin/Hippo pathway; Authors - Chao Zhang

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