Anticorps Monoclonal anti-LIG4

LIG4 Monoclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IF,ELISA

Conjugaison

Non conjugué

CloneNo.

1H6C11

N° de cat : 66705-1-Ig

Synonymes

DNA ligase 4, DNA ligase IV, LIG4, ligase IV, DNA, ATP dependent

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Galerie de données de validation

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  WB analysis of PC-3 using 66705-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66705-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66705-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66705-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 66705-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 66705-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 66705-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66705-1-Ig (LIG4 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules HeLa, cellules HepG2, cellules Jurkat, cellules Ramos, tissu testiculaire humain
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66705-1-Ig cible LIG4 dans les applications de WB, IF,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LIG4 Protéine recombinante Ag3385
• Nom complet: ligase IV, DNA, ATP-dependent
• Masse moléculaire calculée: 911 aa, 104 kDa
• Poids moléculaire observé: 100-104 kDa
• Numéro d’acquisition GenBank: BC037491
• Symbole du gène: LIG4
• Identification du gène (NCBI): 3981
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand brdownloadeaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4.