Anticorps Polyclonal de lapin anti-LGI3

LGI3 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 21919-1-AP

Synonymes

LGI1 like protein 4, LGI3, LGIL4

---

Galerie de données de validation

• 

  WB analysis of HepG2 using 21919-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21919-1-AP (LGI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 21919-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21919-1-AP (LGI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 21919-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21919-1-AP (LGI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 21919-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21919-1-AP (LGI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21919-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21919-1-AP (LGI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 21919-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 21919-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 21919-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 21919-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 21919-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 21919-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 21919-1-AP

  Immunofluorescent analysis of HepG2 cells using 21919-1-AP (LGI3 Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-Rabbit IgG (red).

---

Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A549, cellules SMMC-7721, tissu hépatique de souris, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu pulmonaire humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

21919-1-AP cible LGI3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LGI3 Protéine recombinante Ag16257
• Nom complet: leucine-rich repeat LGI family, member 3
• Masse moléculaire calculée: 548 aa, 62 kDa
• Poids moléculaire observé: 55-62 kDa
• Numéro d’acquisition GenBank: BC142997
• Symbole du gène: LGI3
• Identification du gène (NCBI): 203190
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF	Am J Hum Genet; A reverse genetics and genomics approach to gene paralog function and disease: Myokymia and the juxtaparanode; Authors - Dana Marafi