Anticorps Polyclonal de lapin anti-LETM1

• Phare
• Validé par KD/KO

LETM1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (1)

N° de cat : 16024-1-AP

Synonymes

LETM1

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Galerie de données de validation

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  WB analysis using 16024-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16024-1-AP (LETM1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 16024-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16024-1-AP (LETM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16024-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16024-1-AP (LETM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 16024-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 16024-1-AP (LETM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 16024-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 16024-1-AP (LETM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16024-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16024-1-AP (LETM1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

• 

  IF Staining of HeLa using 16024-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LETM1 antibody (16024-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules Ramos
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de l'œsophage humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

16024-1-AP cible LETM1 dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LETM1 Protéine recombinante Ag8861
• Nom complet: leucine zipper-EF-hand containing transmembrane protein 1
• Masse moléculaire calculée: 739 aa, 83 kDa
• Poids moléculaire observé: 83 kDa
• Numéro d’acquisition GenBank: BC021208
• Symbole du gène: LETM1
• Identification du gène (NCBI): 3954
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LETM1 is a mitochondrial inner membrane protein. It functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. It served as an anchor protein for complex formation between mitochondria and ribosome, and regulated mitochondrial biogenesis. It was also identified inWolf-Hirschhorn syndrome, and was deleted in nearly all patients with the syndrome. A recent study determined that adenovirus-mediated LETM1 may provide a useful target for designing lung tumor prevention and treatment.

Publications

Species	Application	Title
mouse	WB	Nature; The mitochondrial Na(+)/Ca(2+) exchanger is essential for Ca(2+) homeostasis and viability.; Authors - Timothy S Luongo
human	WB	Sci Adv; A common genetic variant of a mitochondrial RNA processing enzyme predisposes to insulin resistance.; Authors - Giulia Rossetti
mouse	WB	Nat Commun; Mutations in Hcfc1 and Ronin result in an inborn error of cobalamin metabolism and ribosomopathy.; Authors - Tiffany Chern
mouse	WB	Proc Natl Acad Sci U S A; Letm1, the mitochondrial Ca2+/H+ antiporter, is essential for normal glucose metabolism and alters brain function in Wolf-Hirschhorn syndrome.; Authors - Jiang Dawei D ; KO Validated
mouse	WB	Cell Rep; The Mitochondrial Calcium Uniporter Matches Energetic Supply with Cardiac Workload during Stress and Modulates Permeability Transition.; Authors - Timothy S Luongo
human	WB	Genes Dis; Aberrant LETM1 elevation dysregulates mitochondrial functions and energy metabolism and promotes lung metastasis in osteosarcoma; Authors - Yulu Shi ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

SITING (Verified Customer) (08-31-2020)

easy to expose

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mice heart

LETM1 Antibody Western Blot validation (1:1000 dilution) in mice heart (Cat no:16024-1-AP)