Anticorps Polyclonal de lapin anti-P3H2

• Phare
• Validé par KD/KO

P3H2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 15723-1-AP

Synonymes

leprecan like 1, Leprecan like protein 1, LEPREL1, MLAT4, P3H2, Prolyl 3 hydroxylase 2

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Galerie de données de validation

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  WB analysis using 15723-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15723-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 15723-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15723-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 15723-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 15723-1-AP (P3H2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 15723-1-AP

  IP result of anti-P3H2 (IP:15723-1-AP, 4ug; Detection:15723-1-AP 1:600) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human liver using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15723-1-AP (P3H2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15723-1-AP (P3H2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15723-1-AP (P3H2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15723-1-AP (P3H2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 15723-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15723-1-AP (P3H2 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules L02, cellules HEK-293, tissu placentaire de souris, tissu placentaire humain, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu cérébral humain, tissu cutané humain, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below

Informations sur le produit

15723-1-AP cible P3H2 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: P3H2 Protéine recombinante Ag8444
• Nom complet: leprecan-like 1
• Masse moléculaire calculée: 708aa,81 kDa; 527aa,60 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC005029
• Symbole du gène: LEPREL1
• Identification du gène (NCBI): 55214
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

P3H2 (prolyl 3-hydroxylase 2, also known as LEPREL1) is a member of the leprecan family of proteins, which also include P3H1, P3H3, CRTAP and SC56. Collagen prolyl hydroxylases are required for proper collagen biosynthesis, folding, and assembly. P3H2 shows prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types II, IV and V. Mutations in this gene are associated with nonsyndromic severe myopia with cataract and vitreoretinal degeneration, and downregulation of this gene may play a role in breast cancer.

Publications

Species	Application	Title
mouse	WB	J Biol Chem; Post-translationally abnormal collagens of prolyl 3-hydroxylase-2 null mice offer a pathobiological mechanism for the high myopia linked to human LEPREL1 mutations*; Authors - David M Hudson
human	WB	Sci Rep; Transcriptome analysis of a dog model of congestive heart failure shows that collagen-related 2-oxoglutarate-dependent dioxygenases contribute to heart failure; Authors - Takahiro Isono
human	WB	J Biol Chem; Post-translational modification of type IV collagen with 3-hydroxyproline affects its interactions with glycoprotein VI and nidogens 1 and 2.; Authors - Nathan T Montgomery ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shinford (Verified Customer) (10-26-2023)

We conducted a screening of basal expression levels in multiple human tumor cell lines, attempting antibody concentrations ranging from 1:500 to 2000, all of which exhibited clear bands, indicating stable working conditions. However, the expression depends on the cancer cell line itself.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Multiple cancer cell lines

P3H2 Antibody Western Blot validation (1:500 dilution) in Multiple cancer cell lines (Cat no:15723-1-AP)