Anticorps Polyclonal de lapin anti-LEO1

LEO1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 12281-1-AP

Synonymes

LEO1, RDL

---

Galerie de données de validation

• 

  WB analysis of mouse heart using 12281-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12281-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 12281-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12281-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12281-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 12281-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12281-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12281-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12281-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12281-1-AP (LEO1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 12281-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12281-1-AP (LEO1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 12281-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12281-1-AP (LEO1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 12281-1-AP

  Immunofluorescent analysis of Hela cells, using LEO1 antibody 12281-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HeLa, cellules HL-60, cellules Jurkat, tissu cérébral de souris, tissu de muscle squelettique de souris, tissu placentaire humain
• Résultats positifs en IHC: tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

12281-1-AP cible LEO1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LEO1 Protéine recombinante Ag2932
• Nom complet: Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
• Masse moléculaire calculée: 666 aa, 75 kDa
• Poids moléculaire observé: 105 kDa
• Numéro d’acquisition GenBank: BC018147
• Symbole du gène: LEO1
• Identification du gène (NCBI): 123169
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LEO1, also named as RNA polymerase-associated protein LEO1, is a 666 amino acid protein, which belongs to the LEO1 family. LEO1 is a component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. The calculated molecular weight of LEO1 is 75 kDa, but the modified LEO1 protein is about 105 kDa (PMID: 15632063).

Publications

Species	Application	Title
human	WB	Nat Neurosci; Toxic expanded GGGGCC repeat transcription is mediated by the PAF1 complex in C9orf72-associated FTD.; Authors - Lindsey D Goodman
mouse	WB	Cell Death Dis; UBR5 targets tumor suppressor CDC73 proteolytically to promote aggressive breast cancer.; Authors - Gang Xiang
human	WB	Sci Adv; CDK12 and Integrator-PP2A complex modulates LEO1 phosphorylation for processive transcription elongation; Authors - Min Qiu
mouse	WB,IP	Cell; Inherited blood cancer predisposition through altered transcription elongation; Authors - Jiawei Zhao