Anticorps Polyclonal de lapin anti-LENG9

LENG9 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 16295-1-AP

Synonymes

LENG9

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Galerie de données de validation

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  WB analysis of HeLa using 16295-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16295-1-AP (LENG9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 16295-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 16295-1-AP (LENG9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16295-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 16295-1-AP

  Immunofluorescent analysis of Hela cells, using LENG9 antibody 16295-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of MCF-7 using 16295-1-AP

  Immunofluorescent analysis of MCF-7 cells, using LENG9 antibody 16295-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Y79
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque humain, tissu cérébral humain, tissu cutané humain, tissu hépatique humain, tissu placentaire humain, tissu pulmonaire humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

16295-1-AP cible LENG9 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LENG9 Protéine recombinante Ag9481
• Nom complet: leukocyte receptor cluster (LRC) member 9
• Masse moléculaire calculée: 501 aa, 53 kDa
• Poids moléculaire observé: 54-60 kDa
• Numéro d’acquisition GenBank: BC015921
• Symbole du gène: LENG9
• Identification du gène (NCBI): 94059
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LENG9 is rarely reported in the literature. GO function analysis revealed that the molecular function of LENG9 is metal ion binding (GO:0046872), and the BioGRID database showed that LENG9 has 6 interactors, including C9ORF41, B4GALT2, CDC5L, D2HGDH, FOXS1 and OGT. Studies have shown that LENG9 may play an oncogenic role in tumor pathogenesis by promoting tumor cell proliferation, migration, and invasion. (PMID: 35538075)

Publications

Species	Application	Title
human	WB	Nat Commun; Long-read sequencing unveils high-resolution HPV integration and its oncogenic progression in cervical cancer.; Authors - Liyuan Zhou