Anticorps Polyclonal de lapin anti-LDHB-Specific

• Phare
• Validé par KD/KO

LDHB-Specific Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 19988-1-AP

Synonymes

lactate dehydrogenase B, LDH B, LDH H, LDH heart subunit, LDHB, LDH-B, LDHB-Specific, TRG 5

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Galerie de données de validation

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  WB analysis using 19988-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19988-1-AP (LDHB-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19988-1-AP

  WB result of LDHB-Specific antibody (19988-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-LDHB-Specific transfected HeLa cells.

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  IF Staining of HeLa using 19988-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LDHB-Specific antibody (19988-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules DU 145, cellules HEK-293T, cellules HeLa, cellules Jurkat, cellules PC-3, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

19988-1-AP cible LDHB-Specific dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: lactate dehydrogenase B
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: NM_002300
• Symbole du gène: LDHB
• Identification du gène (NCBI): 3945
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LDHB, also named as NY-REN-46 and LDH-H, belongs to the LDH/MDH superfamily and LDH family. LDHB catalyzes the reaction: (S)-lactate + NAD+ = pyruvate + NADH. It is an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway. It is glycolytic enzymes and it is positively regulated by mTOR.Defects in LDHB are a cause of hereditary LDHB deficiency. The antibody is specific to LDHB and has no cross reaction with LDHA and LDHC.

Publications

Species	Application	Title
mouse	WB	Biomedicines; HMTM-Mediated Enhancement of Brain Bioenergetics in a Mouse Tauopathy Model Is Blocked by Chronic Administration of Rivastigmine.; Authors - Renato X Santos
mouse	WB	J Nutr Sci Vitaminol (Tokyo); Combination of Exercise and Intake of Amino Acid Mixture Synergistically Induces Beige Adipocyte Formation in Mice; Authors - Takuya Kojima
human	WB	Mol Biol Rep; Development of a one-plasmid system to replace the endogenous protein with point mutation for post-translational modification studies.; Authors - Iqra Ishrat
mouse	IHC, IF	Am J Physiol Renal Physiol; Expression of lactate dehydrogenase A and B isoforms in the mouse kidney.; Authors - Gunars Osis
human	WB,IF	iScience; Lactate-driven type I collagen deposition facilitates cancer stem cell-like phenotype of head and neck squamous cell carcinoma; Authors - Yunqing Sun ; KD Validated