Anticorps Polyclonal de lapin anti-LBP

LBP Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 23559-1-AP

Synonymes

LBP

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Galerie de données de validation

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  WB analysis of mouse liver using 23559-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23559-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 23559-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23559-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 23559-1-AP

  IP result of anti-LBP (IP:23559-1-AP, 4ug; Detection:23559-1-AP 1:500) with mouse liver tissue lysate 3040 ug.

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

23559-1-AP cible LBP dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LBP Protéine recombinante Ag13485
• Nom complet: lipopolysaccharide binding protein
• Masse moléculaire calculée: 481 aa, 53 kDa
• Poids moléculaire observé: 53-60 kDa
• Numéro d’acquisition GenBank: BC022256
• Symbole du gène: LBP
• Identification du gène (NCBI): 3929
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LBP (lipopolysaccharide-binding protein) is an acute-phase protein, predominantly synthesized by hepatocytes. It is present in normal serum at concentrations of 5 to 10 μg/ml, rising up to 200 μg/ml 24 h after induction of an acute-phase response. LBP binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS. (PMID: 9665271; 11598069)

Publications

Species	Application	Title
mouse	WB	Antioxidants (Basel); Antioxidative Self-Assembling Nanoparticles Attenuate the Development of Steatohepatitis and Inhibit Hepatocarcinogenesis in Mice; Authors - Takahisa Watahiki
human	WB	Front Microbiol; Proteomics Analysis of Exosomes From Patients With Active Tuberculosis Reveals Infection Profiles and Potential Biomarkers.; Authors - Min Zhang
bovine	WB	J Proteomics; Characterization of bovine mammary gland dry secretions and their proteome from the end of lactation through day 21 of the dry period.; Authors - Timothy A Reinhardt
human,rat	IHC	Nutrition; Branched-chain amino acids protect the liver from cirrhotic injury via suppression of activation of lipopolysaccharide-binding protein, toll-like receptor 4, and signal transducer and activator of transcription 3, as well as Enterococcus faecalis translocation; Authors - Akiko Eguchi
mouse	WB,IF	Eur J Pharmacol; Chromofungin, a chromogranin A-derived peptide, protects against sepsis-induced acute lung injury by inhibiting LBP/TLR4-dependent inflammatory signaling; Authors - Wushuang Zhou
mouse	WB	PLoS One; Macrophage specific restoration of the Nrf2 gene in whole-body knockout mice ameliorates steatohepatitis induced by lipopolysaccharide from Porphyromonas gingivalis through enhanced hepatic clearance; Authors - Kanako Chihara