Anticorps Polyclonal de lapin anti-LASS2

• Phare
• Validé par KD/KO

LASS2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 20344-1-AP

Synonymes

CerS2, L3, LASS2, SP260, TMSG1

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Galerie de données de validation

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  WB analysis using 20344-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20344-1-AP (LASS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20344-1-AP

  WB result of LASS2 antibody (20344-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-LASS2 transfected HeLa cells.

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  WB analysis using 20344-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20344-1-AP (LASS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20344-1-AP

  IP result of anti-LASS2 (IP:20344-1-AP, 4ug; Detection:20344-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human liver using 20344-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20344-1-AP (LASS2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 20344-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20344-1-AP (LASS2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse lung using 20344-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20344-1-AP (LASS2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse lung using 20344-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20344-1-AP (LASS2 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HeLa, cellules HepG2, tissu hépatique de rat
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu hépatique humain, tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

20344-1-AP cible LASS2 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LASS2 Protéine recombinante Ag14151
• Nom complet: LAG1 homolog, ceramide synthase 2
• Masse moléculaire calculée: 380 aa, 45 kDa
• Poids moléculaire observé: 45 kDa, 37 kDa
• Numéro d’acquisition GenBank: BC010032
• Symbole du gène: LASS2
• Identification du gène (NCBI): 29956
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LASS2, also known as TMSG1, is a novel suppressor of human cancer metastasis. As one member of LASS family, including LASS1-6, LASS2 mRNA is at the highest level of all LASS members, and has the broadest tissue distribution, particularly abundant in the liver, kidney and brain in mice. The biological roles of LASS2 include protection from aging , hepatic INS resistance, and hepatocellular carcinoma (HCC) progression. LASS2 has been correlated with the degree of invasion and recurrence of carcinomas of the prostate, liver, breast and bladder.

Publications

Species	Application	Title
mouse	WB	Cardiovasc Res; Nogo-A reduces ceramide de novo biosynthesis to protect from heart failure.; Authors - Linda Sasset ; KD Validated
human	WB,IHC,IF	J Cancer; LASS2 impairs proliferation of glioma stem cells and migration and invasion of glioma cells mainly via inhibition of EMT and apoptosis promotion.; Authors - Wei-Jiang Zhao ; KD Validated
human	WB	Exp Cell Res; MicroRNA-98 promotes drug resistance and regulates mitochondrial dynamics by targeting LASS2 in bladder cancer cells.; Authors - Ting Luan ; KD Validated
human	WB,IF	J Cancer; LASS2 regulates invasion and chemoresistance via ERK/Drp1 modulated mitochondrial dynamics in bladder cancer cells.; Authors - Lijuan Huang ; KD Validated
human	WB	Tumour Biol; miR-9 promotes cell proliferation and inhibits apoptosis by targeting LASS2 in bladder cancer.; Authors - Haifeng Wang