Anticorps Polyclonal de lapin anti-LARP1

• Phare
• Validé par KD/KO

LARP1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Drosophile, Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 13708-1-AP

Synonymes

KIAA0731, La related protein 1, LARP, LARP1

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Galerie de données de validation

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  WB analysis of HEK-293T using 13708-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 13708-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13708-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13708-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13708-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13708-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13708-1-AP (LARP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 13708-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13708-1-AP (LARP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 13708-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13708-1-AP (LARP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 13708-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13708-1-AP (LARP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13708-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13708-1-AP (LARP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 13708-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 13708-1-AP (LARP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 13708-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LARP1 antibody (13708-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules HepG2, tissu cardiaque de souris, tissu cérébral de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below

Informations sur le produit

13708-1-AP cible LARP1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Drosophile, Humain, rat, souris

• Réactivité: Drosophile, Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LARP1 Protéine recombinante Ag4645
• Nom complet: La ribonucleoprotein domain family, member 1
• Masse moléculaire calculée: 1096 aa, 124 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: BC033856
• Symbole du gène: LARP1
• Identification du gène (NCBI): 23367
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Sci Adv; The 40S-LARP1 complex reprograms the cellular translatome upon mTOR inhibition to preserve the protein synthetic capacity.; Authors - Pedro Fuentes ; KO Validated
human	WB	Mol Cell Proteomics; A new cellular interactome of SARS-CoV-2 nucleocapsid protein and its biological implications; Authors - Yuan-Qin Min
mouse	WB	Mol Autism; Ribosome profiling in mouse hippocampus: plasticity-induced regulation and bidirectional control by TSC2 and FMRP.; Authors - Annie Hien