Anticorps Polyclonal de lapin anti-LARG

• Phare
• Validé par KD/KO

LARG Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 22441-1-AP

Synonymes

ARHGEF12, KIAA0382, LARG, Leukemia associated RhoGEF, PRO2792

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Galerie de données de validation

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  WB analysis of Jurkat using 22441-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22441-1-AP (LARG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22441-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22441-1-AP (LARG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 22441-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22441-1-AP (LARG Antibody) at dilution of 1:50.

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  IHC staining of human testis using 22441-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22441-1-AP (LARG Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 22441-1-AP

  Immunofluorescent analysis of HepG2 cells, using ARHGEF12 antibody 22441-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 22441-1-AP

  Immunofluorescent analysis of HeLa cells using 22441-1-AP (LARG antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa
• Résultats positifs en IHC: tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

22441-1-AP cible LARG dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LARG Protéine recombinante Ag18211
• Nom complet: Rho guanine nucleotide exchange factor (GEF) 12
• Masse moléculaire calculée: 1544 aa, 173 kDa
• Poids moléculaire observé: 220 kDa
• Numéro d’acquisition GenBank: BC063117
• Symbole du gène: LARG
• Identification du gène (NCBI): 23365
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LARG, also known as ARHGEF12, is one of three regulator of G protein signalling (RGS) domain-containing RhoGEFs. LARG activates the ROCK pathway downstream of Gα12/13 signaling, leading to cytoskeletal reorganisation. LARG performs this activity either as a homodimer or as a heterodimer with Arhgef11. LARG has been implicated in many diseases, including cancer, heart disease, and in HIV viral replication. LARG is phosphorylated and dephosphorylated during mitosis and that Cdk1 is the mitotic kinase responsible for LARG phosphorylation. We got 220 kDa in western blotting, maybe due to its phosphorylation.

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; RSK2 drives cell motility by serine phosphorylation of LARG and activation of Rho GTPases.; Authors - Geng-Xian Shi
human	WB	Br J Pharmacol; Reregulation of hepatic stellate cell contraction and cirrhotic portal hypertension by Wnt/β-catenin signaling via interaction with Gli1.; Authors - Feng Zhang
human	WB,IHC	Am J Pathol; The Guanine Nucleotide Exchange Factor, Leukemia-Associated Rho Guanine Nucleotide Exchange Factor, and RhoC Play a Role in Glioblastoma Cell Invasion and Resistance.; Authors - Zonghui Ding ; KD Validated
mouse	WB	Int J Mol Sci; Inhibition of High-Temperature Requirement Protein A2 Protease Activity Represses Myogenic Differentiation via UPRmt; Authors - Hongyu Sun