Anticorps Polyclonal de lapin anti-LAPTM4B

• Phare
• Validé par KD/KO

LAPTM4B Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 18895-1-AP

Synonymes

LAPTM4B, LAPTM4beta, LC27

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Galerie de données de validation

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  WB analysis of mouse lymph using 18895-1-AP

  mouse lymph tissue were subjected to SDS PAGE followed by western blot with 18895-1-AP (LAPTM4B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lymph using 18895-1-AP

  rat lymph tissue were subjected to SDS PAGE followed by western blot with 18895-1-AP (LAPTM4B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 18895-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18895-1-AP (LAPTM4B Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 18895-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18895-1-AP (LAPTM4B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18895-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18895-1-AP (LAPTM4B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 18895-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18895-1-AP (LAPTM4B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 18895-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18895-1-AP (LAPTM4B Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu lymphatique de souris, tissu lymphatique de rat
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Informations sur le produit

18895-1-AP cible LAPTM4B dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LAPTM4B Protéine recombinante Ag13521
• Nom complet: lysosomal protein transmembrane 4 beta
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC014129
• Symbole du gène: LAPTM4B
• Identification du gène (NCBI): 55353
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LAPTM4B, a novel oncoprotein, is associated with the prognosis of cancer, and may play important roles in the initiation, promotion and metastasis of tumors. It was initially identified as a hepatocellular carcinoma-associated gene, and was recently shown to be up-regulated in various human cancers. Some studies indicated that LAPTM4B may be an useful marker of prognosis in ovarian carcinoma and endometrial cancer. LAPTM4B belongs to the LAPTM4/LAPTM5 transporter family. It has 3 isoforms with molecular masses 41 kDa, 35 kDa and 24 kDa. This antibody can recognize all the 3 isoforms of LAPTM4B.

Publications

Species	Application	Title
human	WB, IF	Cell; A kinase-independent role for EGF receptor in autophagy initiation.; Authors - Xiaojun Tan ; KD Validated
	WB	Nat Commun; LAPTM4b recruits the LAT1-4F2hc Leu transporter to lysosomes and promotes mTORC1 activation.; Authors - Ruth Milkereit
human	IF	Proc Natl Acad Sci U S A; PtdIns(4,5)P2 signaling regulates ATG14 and autophagy.; Authors - Xiaojun Tan
	IHC	Front Cell Dev Biol; Integrative Genome-Wide DNA Methylome and Transcriptome Analysis of Ovaries from Hu Sheep with High and Low Prolific.; Authors - Xiaolei Yao
human	WB,IHC,IF	Biochem Biophys Res Commun; Helicobacter pylori infection promotes epithelial-to-mesenchymal transition of gastric cells by upregulating LAPTM4B.; Authors - Shengfei Zhou ; KD Validated
human	IF	Cancer Manag Res; LAPTM4B facilitates tumor growth and induces autophagy in hepatocellular carcinoma.; Authors - Fei Wang