Anticorps Polyclonal de lapin anti-LAMR1,RPSA

• Phare
• Validé par KD/KO

LAMR1,RPSA Polyclonal Antibody for WB, IF, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 14533-1-AP

Synonymes

37/67 kDa laminin receptor, 37LRP, 40S ribosomal protein SA, 67 kDa laminin receptor, 67LR, LAMBR, Laminin receptor, Laminin receptor 1, LamR, LAMR1, LAMR1,RPSA, LBP/p40, LRP, LRP/LR, NEM/1CHD4, p40, ribosomal protein SA, RPSA

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Galerie de données de validation

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  WB analysis using 14533-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14533-1-AP (LAMR1,RPSA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14533-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14533-1-AP (LAMR1,RPSA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 14533-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14533-1-AP (LAMR1,RPSA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 14533-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LAMR1,RPSA antibody (14533-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 14533-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human LAMR1,RPSA (14533-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HeLa, cellules NIH/3T3, cellules PC-12, tissu de côlon de souris, tissu hépatique de souris
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

14533-1-AP cible LAMR1,RPSA dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LAMR1,RPSA Protéine recombinante Ag6033
• Nom complet: ribosomal protein SA
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC050688
• Symbole du gène: RPSA
• Identification du gène (NCBI): 3921
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The ribosomal protein SA (RPSA), previously named 67 kD laminin receptor(67LR), 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein, is a multifunctional protein, that plays a role in a number of pathological processes, such as cancer and prion diseases. It is overexpressed in various cancer cell lines, and the the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. This antibody is a rabbit polyclonal antibody raised against full length RPSA of human origin. This antibody is specific to RPSA (LAMR1).67LR derives from homo- or hetero- dimerization of a 37 kDa cytosolic precursor (37LRP)], most probably by fatty acid acylation. 37LRP is mostly found in the cytosol and nucleus [10] where it is involved in translational processes and maintenance of nuclear structures, respectively. 67LR is localized at the cell surface and it not only serves as a receptor for LM but also acts as a receptor for elastin, carbohydrates and the cellular prion protein.

Publications

Species	Application	Title
	IF	Neuron; Rapid Cue-Specific Remodeling of the Nascent Axonal Proteome.; Authors - Roberta Cagnetta
mouse	WB	Arterioscler Thromb Vasc Biol; β1-Integrin Is Essential for Vasoregulation and Smooth Muscle Survival In Vivo.; Authors - Turlo Kirsten A KA
human	WB	Virulence; LAMR1 restricts Zika virus infection by attenuating the envelope protein ubiquitination.; Authors - Dingwen Hu ; KD Validated
mouse	WB	Hum Gene Ther; Strategies to Attenuate Myocardial Infarction and No-Reflow through Preservation of Vascular Integrity by Pigment Epithelium-derived Factor.; Authors - Hao Zhang
mouse	WB	Sci Rep; Epigallocatechin-3-gallate inhibits H2O2-induced apoptosis in Mouse Vascular Smooth Muscle Cells via 67kD Laminin Receptor.; Authors - Xue Yan
human,mouse	WB,IHC	Food Funct; Theaflavin-3,3'-di-gallate represses prostate cancer by activating the PKCδ/aSMase signaling pathway through a 67 kDa laminin receptor.; Authors - Lingli Sun