Anticorps Polyclonal de lapin anti-Laminin beta 1

Laminin beta 1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

24

N° de cat : 23498-1-AP

Synonymes

LAMB1, CLM, Laminin 2 subunit beta, Laminin 12 subunit beta, Laminin 10 subunit beta

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 23498-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23498-1-AP (LAMB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 23498-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23498-1-AP (LAMB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 23498-1-AP

  IP result of anti-Laminin beta 1 (IP:23498-1-AP, 4ug; Detection:23498-1-AP 1:2000) with NIH/3T3 cells lysate 1920 ug.

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  IF Staining of HepG2 using 23498-1-AP

  Immunofluorescent analysis of HepG2 cells, using LAMB1 antibody 23498-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules Jurkat
• Résultats positifs en IP: cellules NIH/3T3,
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 12 publications below

Informations sur le produit

23498-1-AP cible Laminin beta 1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Laminin beta 1 Protéine recombinante Ag19182
• Nom complet: laminin, beta 1
• Masse moléculaire calculée: 1786 aa, 198 kDa
• Poids moléculaire observé: 220 kDa
• Numéro d’acquisition GenBank: BC113455
• Symbole du gène: Laminin beta 1
• Identification du gène (NCBI): 3912
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Laminins are a family of basement membrane glycoproteins composed of three nonidentical chains, laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) (PMID: 7959779). Laminins contribute to the structure of extracellular matrix and influence the behavior of associated cells, such as adhesion, differentiation, migration, phenotype stability, and resistance to anoikis (PMID: 23057746). Laminin beta 1 (LAMB1) is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin-1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. Laminin beta 1 is also a subunit of laminin-2 (laminin-211 or merosin), laminin-6 (laminin-311 or K-laminin), laminin-8 (laminin-411), laminin-10 (laminin-511) and laminin-12 (laminin-213).

Publications

Species	Application	Title
rat	WB	Acta Pharm Sin B; A novel PGAM5 inhibitor LFHP-1c protects blood-brain barrier integrity in ischemic stroke.; Authors - Chenglong Gao
mouse	IF	ACS Appl Mater Interfaces; Novel Thermosensitive Hydrogel Promotes Spinal Cord Repair by Regulating Mitochondrial Function.; Authors - Yi Li
human	WB	Cell Rep; Stub1 maintains proteostasis of master transcription factors in embryonic stem cells.; Authors - Md Mahfuz Al Mamun
mouse	IF	J Neuroinflammation; SU16f inhibits fibrotic scar formation and facilitates axon regeneration and locomotor function recovery after spinal cord injury by blocking the PDGFRβ pathway.; Authors - Ziyu Li
rat	IF,WB	Brain Pathol; Reconstituting neurovascular unit with primary neural stem cells and brain microvascular endothelial cells in three-dimensional matrix.; Authors - Hongjin Wang
human	WB	Angiogenesis; SNAI1, an endothelial-mesenchymal transition transcription factor, promotes the early phase of ocular neovascularization.; Authors - Jia-Xing Sun