Anticorps Polyclonal de lapin anti-LACTB

• Phare
• Validé par KD/KO

LACTB Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 18195-1-AP

Synonymes

G24, lactamase, beta, LACTB, MRPL56

---

Galerie de données de validation

• 

  WB analysis of HepG2 using 18195-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18195-1-AP (LACTB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 18195-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18195-1-AP (LACTB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18195-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18195-1-AP (LACTB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 18195-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18195-1-AP (LACTB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 18195-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18195-1-AP (LACTB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 18195-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18195-1-AP (LACTB antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 18195-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18195-1-AP (LACTB antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 18195-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 18195-1-AP (LACTB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, tissu cardiaque humain
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 5 publications below
• IF: See 4 publications below

Informations sur le produit

18195-1-AP cible LACTB dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LACTB Protéine recombinante Ag12233
• Nom complet: lactamase, beta
• Masse moléculaire calculée: 547 aa, 61 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: BC067288
• Symbole du gène: LACTB
• Identification du gène (NCBI): 114294
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LACTB is also named as MRPL56,which is a component of the large subunit of the mitochondrial ribosome that is encoded by the nuclear genome.LACTB is a mammalian active-site serine protein that has evolved from a bacterial penicillin-binding protein.It promotes intramitochondrial membrane organization and micro-compartmentalization through polymerization(PMID:19858488). Northern blot analysis of mouse tissues detected expression at high levels in liver, heart, and skeletal muscle, with lower levels in kidney, testis, brain, lung, and spleen(PMID:11707067). The full-length of LACTB is about 61kDa, and a 115aa transit peptide can be removed in the mature form. This antibody is specific to LACTB.

Publications

Species	Application	Title
human	WB,IHC,IF	Nature; LACTB is a tumour suppressor that modulates lipid metabolism and cell state.; Authors - Zuzana Keckesova
human	WB,IHC,IF	Oncogene; LACTB, a novel epigenetic silenced tumor suppressor, inhibits colorectal cancer progression by attenuating MDM2-mediated p53 ubiquitination and degradation.; Authors - Kaixuan Zeng ; KO Validated
Human	IHC	PLoS One; LACTB mRNA expression is increased in pancreatic adenocarcinoma and high expression indicates a poor prognosis.; Authors - Jian Xie
human	IHC	Cancer Biother Radiopharm; Clinical Significance of β-Lactamase Expression in Colorectal Cancer.; Authors - Shaofei Zhou
human	WB	Exp Ther Med; LACTB suppresses carcinogenesis in lung cancer and regulates the EMT pathway.; Authors - Yihui Xu ; KD Validated
human	WB,IF	Apoptosis; LACTB induces cancer cell death through the activation of the intrinsic caspase-independent pathway in breast cancer; Authors - Juan M Gonzalez-Morena