• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-L2HGDH

L2HGDH Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 15707-1-AP

Synonymes

C14orf160, DURANIN, L2HGDH



Applications testées

Résultats positifs en WBcellules A549, cellules MCF-7, cellules SGC-7901, tissu cérébral de rat, tissu d'intestin grêle de souris
Résultats positifs en IHCtissu de cancer du foie humain, tissu de gliome humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochimie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

15707-1-AP cible L2HGDH dans les applications de WB, FC, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène L2HGDH Protéine recombinante Ag8382
Nom complet L-2-hydroxyglutarate dehydrogenase
Masse moléculaire calculée463aa,50 kDa; 441aa,49 kDa
Poids moléculaire observé 45 kDa
Numéro d’acquisition GenBankBC006117
Symbole du gène L2HGDH
Identification du gène (NCBI) 79944
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

L2HGDH(L-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as duranin, C14orf160 and belongs to the L2HGDH family. The putative L2HGDH is predicted to be targeted to the mitochondria where its mitochondrial targeting sequence is presumably removed(PMID:16005139). Defects in L2HGDH are the cause of L-2-hydroxyglutaric aciduria (L2HGA). It has 2 isoforms produced by alternative splicing with the molecular weight of 50 kDa and 48 kDa. L2HGDH also can be detected as ~45kD due to the 51aa transit peptide cleaved.

Protocole

Product Specific Protocols
WB protocol for L2HGDH antibody 15707-1-APDownload protocol
IHC protocol for L2HGDH antibody 15707-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

Hypoxia Induces Production of L-2-Hydroxyglutarate.

Authors - Andrew M Intlekofer
  • KD Validated
humanWB

Cell Metab

Hypoxia-Mediated Increases in L-2-hydroxyglutarate Coordinate the Metabolic Response to Reductive Stress.

Authors - William M Oldham
  • KD Validated
humanWB

Sci Transl Med

2-Hydroxyglutarate produced by neomorphic IDH mutations suppresses homologous recombination and induces PARP inhibitor sensitivity.

Authors - Parker L Sulkowski
  • KD Validated
humanWB

Cancer Discov

L-2-Hydroxyglutarate: an epigenetic modifier and putative oncometabolite in renal cancer.

Authors - Eun-Hee Shim
humanWB

Cell Chem Biol

MYC Regulation of D2HGDH and L2HGDH Influences the Epigenome and Epitranscriptome.

Authors - ZhiJun Qiu
  • KO Validated

Clin Cancer Res

Biochemical and Epigenetic Insights into L-2-Hydroxyglutarate, a Potential Therapeutic Target in Renal Cancer.

Authors - Sandeep Shelar

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Anirban (Verified Customer) (12-05-2021)

24 ug protein/lane. One non specific band observed above the KD band. 5% milk was used to block (1 hr incubation) and in secondary Ab (1/5000 dilution-45 minutes incubation). Primary Ab was diluted in 3% BSA (1.5 hrs incubation. All were done in TBST (0.1% Tween 20). Nitrocellulose membrane was used.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Renca (mouse renal cancer cell line)
L2HGDH Antibody Western Blot validation (1/1000 dilution) in Renca (mouse renal cancer cell line) (Cat no:15707-1-AP)
FH

Daniel (Verified Customer) (09-30-2019)

Tissue was boiled in SDS-sample buffer and separated by size on an SDS-PAGE.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse liver
L2HGDH Antibody Western Blot, validation (1:1000 dilution) in Mouse liver (Cat no:15707-1-AP)