Anticorps Polyclonal de lapin anti-L1CAM

L1CAM Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 20659-1-AP

Synonymes

CAML1, CD171, HSAS, HSAS1, L1 cell adhesion molecule, L1CAM, MASA, MIC5, N CAM L1, N CAML1, NCAM L1, S10, SPG1

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Galerie de données de validation

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  WB analysis using 20659-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20659-1-AP (L1CAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

20659-1-AP cible L1CAM dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: L1 cell adhesion molecule
• Masse moléculaire calculée: 140 kDa
• Poids moléculaire observé: 200-220 kDa, 80 kDa
• Numéro d’acquisition GenBank: NM_000425
• Symbole du gène: L1CAM
• Identification du gène (NCBI): 3897
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503). It has been reported that L1CAM can be proteolytically cleaved into a soluble fragment of 140 kDa and an intracellular fragment of 80-85 kDa (PMID: 30842511; 34380733). This antibody raised against 1206-1221aa of human L1CAM detects 200-220 kDa full-length L1CAM and 80-kDa cleaved fragment of L1CAM.

Publications

Species	Application	Title
human	IHC	Histopathology; Frequent KRAS mutations in oncocytic papillary renal neoplasm with inverted nuclei.; Authors - Kuo Tong
human	WB	Diagnostics (Basel); Circulatory Astrocyte and Neuronal EVs as Potential Biomarkers of Neurological Dysfunction in HIV-Infected Subjects and Alcohol/Tobacco Users.; Authors - Sunitha Kodidela
human		Anal Chem; Nanosensor-Driven Detection of Neuron-Derived Exosomal Aβ42 with Graphene Electrolyte-Gated Transistor for Alzheimer's Disease Diagnosis; Authors - Jiahao Li
mouse	WB	Res Sq; Curcumin enhances elvitegravir concentration and alleviates oxidative stress and inflammatory response; Authors - Sandip Godse
mouse	WB	Sci Rep; Curcumin enhances elvitegravir concentration and alleviates oxidative stress and inflammatory response; Authors - Sandip Godse