Anticorps Polyclonal de lapin anti-L-VEGFA

L-VEGFA Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 22341-1-AP

Synonymes

L VEGFA, L-VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF

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Galerie de données de validation

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  WB analysis of MCF-7 using 22341-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 22341-1-AP (L-VEGFA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 22341-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22341-1-AP (L-VEGFA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 22341-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22341-1-AP (L-VEGFA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 22341-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22341-1-AP (L-VEGFA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 22341-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22341-1-AP (L-VEGFA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 22341-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22341-1-AP (L-VEGFA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22341-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22341-1-AP (L-VEGFA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22341-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22341-1-AP (L-VEGFA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules SH-SY5Y
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

22341-1-AP cible L-VEGFA dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: L-VEGFA Protéine recombinante Ag17831
• Nom complet: vascular endothelial growth factor A
• Masse moléculaire calculée: 412 aa, 46 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC065522
• Symbole du gène: VEGFA
• Identification du gène (NCBI): 7422
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1).

Publications

Species	Application	Title
human	WB	Mol Carcinog; PMID: 28833603; Authors - Hui-Hui Zhang
mouse	WB	Life Sci; Circular RNA COL1A2 promotes angiogenesis via regulating miR-29b/VEGF axis in diabetic retinopathy.; Authors - Jing Zou
		Tumour Biol; Evodiamine exerts anti-tumor effects against hepatocellular carcinoma through inhibiting β-catenin-mediated angiogenesis.; Authors - Le Shi