Anticorps Polyclonal de lapin anti-KTN1

• Phare
• Validé par KD/KO

KTN1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 19841-1-AP

Synonymes

CG 1 antigen, CG1, KIAA0004, Kinectin, kinectin 1 (kinesin receptor), Kinesin receptor, KNT, KTN1, MU RMS 40.19

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Galerie de données de validation

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  WB analysis using 19841-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19841-1-AP (KTN1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 19841-1-AP

  WB result of KTN1 antibody (19841-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KTN1 transfected A549 cells.

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  IP experiment of Jurkat using 19841-1-AP

  IP result of anti-KTN1 (IP:19841-1-AP, 4ug; Detection:19841-1-AP 1:500) with Jurkat cells lysate 1200ug.

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  IHC staining of human ovary tumor using 19841-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 19841-1-AP (KTN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 19841-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19841-1-AP (KTN1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 19841-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19841-1-AP (KTN1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 19841-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KTN1 antibody (19841-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 19841-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KTN1 antibody (19841-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules Jurkat, cellules K-562, cellules PC-3, cellules SKOV-3
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

19841-1-AP cible KTN1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KTN1 Protéine recombinante Ag13854
• Nom complet: kinectin 1 (kinesin receptor)
• Masse moléculaire calculée: 1357 aa, 156 kDa
• Poids moléculaire observé: 156 kDa
• Numéro d’acquisition GenBank: BC117132
• Symbole du gène: KTN1
• Identification du gène (NCBI): 3895
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KTN1/Kinectin is an integral endoplasmic reticulum (ER) membrane protein which acts as dynamic anchor to facilitate ER membrane extension along microtubules. It has been found to bind kinesin, Rho GTPase, and translation elongation factor-1delta and gets involved in organelle motility and protein synthesis.

Publications

Species	Application	Title
human,monkey	WB	Nature; ER proteins decipher the tubulin code to regulate organelle distribution; Authors - Pengli Zheng ; KO Validated
human,mouse	WB	Sci Transl Med; CPEB alteration and aberrant transcriptome-polyadenylation lead to a treatable SLC19A3 deficiency in Huntington's disease.; Authors - Sara Picó
human	IF	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
	IF	EMBO Rep; Mobile late endosomes modulate peripheral endoplasmic reticulum network architecture.; Authors - Menno Spits
human	WB, IHC	Clin Sci (Lond); The long noncoding RNA KTN1-AS1 promotes bladder cancer tumorigenesis via KTN1 cis-activation and the consequent initiation of Rho GTPase-mediated signaling.; Authors - Xueying Hu
monkey	WB	Curr Biol; ATL3 Is a Tubular ER-Phagy Receptor for GABARAP-Mediated Selective Autophagy.; Authors - Qingzhou Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Siting (Verified Customer) (01-28-2020)

This antibody worked well with western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue