Anticorps Polyclonal de lapin anti-Keratin 81

• Phare
• Validé par KD/KO

Keratin 81 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 11342-1-AP

Synonymes

ghHb1, ghHkb1, Hair keratin K2.9, Hb 1, HB1, hHAKB2 1, K81, keratin 81, Keratin, hair, basic, 1, Keratin, type II cuticular Hb1, KRT81, KRTHB1, MLN 137, MLN137, Type II hair keratin Hb1, Type II keratin Kb21

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Galerie de données de validation

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  WB analysis using 11342-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11342-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 11342-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 11342-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11342-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11342-1-AP

  IP result of anti-KRT81 (IP:11342-1-AP, 4ug; Detection:11342-1-AP 1:300) with HEK-293 cells lysate 2800ug.

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  IHC staining of human colon cancer using 11342-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11342-1-AP (KRT81 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 11342-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KRT81 antibody (11342-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules MCF-7, tissu de thymus de souris, tissu pancréatique de souris, tissu splénique humain
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

11342-1-AP cible Keratin 81 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Keratin 81 Protéine recombinante Ag1890
• Nom complet: keratin 81
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC021241
• Symbole du gène: KRT81
• Identification du gène (NCBI): 3887
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT81 is a type II keratin.

Publications

Species	Application	Title
human	IHC	Clin Cancer Res; A Preclinical Trial and Molecularly Annotated Patient Cohort Identify Predictive Biomarkers in Homologous Recombination-deficient Pancreatic Cancer.; Authors - Yifan Wang
		Oncol Rep; Hair keratin KRT81 is expressed in normal and breast cancer cells and contributes to their invasiveness.; Authors - Naoki Nanashima
human	WB	Heliyon; Single-cell data analysis of malignant epithelial cell heterogeneity in lung adenocarcinoma for patient classification and prognosis prediction; Authors - Xu Ran ; KD Validated
human,mouse	WB	Adv Sci (Weinh); Ectopic Expression of a Truncated Isoform of Hair Keratin 81 in Breast Cancer Alters Biophysical Characteristics to Promote Metastatic Propensity; Authors - Diane S Kang ; KD Validated