Anticorps Polyclonal de lapin anti-Cytokeratin 13

Cytokeratin 13 Polyclonal Antibody for WB, IP, IF, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 10164-2-AP

Synonymes

CK 13, CK13, Cytokeratin 13, K13, keratin 13, KRT13

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Galerie de données de validation

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  WB analysis of A431 using 10164-2-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10164-2-AP (Cytokeratin 13 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 10164-2-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 10164-2-AP (Cytokeratin 13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 10164-2-AP

  IP result of anti-Cytokeratin 13 (IP:10164-2-AP, 4ug; Detection:10164-2-AP 1:1000) with A431 cells lysate 2400ug.

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  IHC staining of human cervical cancer using 10164-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10164-2-AP (Cytokeratin 13 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human cervical cancer using 10164-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10164-2-AP (Cytokeratin 13 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human oesophagus using 10164-2-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 10164-2-AP (Cytokeratin 13 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human oesophagus using 10164-2-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 10164-2-AP (Cytokeratin 13 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of HaCaT using 10164-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Cytokeratin 13 antibody (10164-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 10164-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 13 antibody (10164-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A431 using 10164-2-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 13 (10164-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, tissu cutané de souris
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu œsophagien humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HaCaT, cellules A431
• Résultats positifs en FC (Intra): cellules A431
• Résultats positifs en cytométrie: cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 7 publications below

Informations sur le produit

10164-2-AP cible Cytokeratin 13 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytokeratin 13 Protéine recombinante Ag0217
• Nom complet: keratin 13
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC002661
• Symbole du gène: Cytokeratin 13
• Identification du gène (NCBI): 3860
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratin 13 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in keratin 13 gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2.

Publications

Species	Application	Title
mouse	IF	Theranostics; Differential effect of cancer-associated fibroblast-derived extracellular vesicles on cisplatin resistance in oral squamous cell carcinoma via miR-876-3p; Authors - Soo Hyun Kang
human	IF	Elife; Chloride channels regulate differentiation and barrier functions of the mammalian airway.; Authors - Mu He
human	WB	Cell Commun Signal; Liprin-α1 modulates cancer cell signaling by transmembrane protein CD82 in adhesive membrane domains linked to cytoskeleton.; Authors - Henna Pehkonen
human	IF	Am J Respir Cell Mol Biol; Do airway epithelium air-liquid cultures represent the in vivo airway epithelium transcriptome?; Authors - Dvorak Anna A
human	IF	Stem Cells Int; Pyridoxal-5'-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-κB Pathway.; Authors - Cong Li
human	IHC	Mol Vis; Expression analysis of human pterygium shows a predominance of conjunctival and limbal markers and genes associated with cell migration.; Authors - Jaworski C J CJ