Anticorps Polyclonal de lapin anti-Cytokeratin 10

Cytokeratin 10 Polyclonal Antibody for WB, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA, FC (Intra)

Conjugaison

Non conjugué

Publications

14

Avis (1)

N° de cat : 18343-1-AP

Synonymes

Cell and organelle markers, CK 10, CK10, Cytokeratin 10, Cytoskeleton Marker, K10, keratin 10, KPP, KRT10

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Galerie de données de validation

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  WB analysis using 18343-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 18343-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 18343-1-PBS

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 18343-1-PBS

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 18343-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18343-1-AP (Cytokeratin 10 antibody) at dilution of 1:200 (under 10x lens).

• 

  FC experiment of A431 using 18343-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 10 (18343-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cutané de souris, cellules A431, tissu cutané de rat
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu cutané de rat, tissu cutané de souris, tissu de cancer de la peau humain, tissu de cancer du poumon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules A431
• Résultats positifs en cytométrie: cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 10 publications below

Informations sur le produit

18343-1-AP cible Cytokeratin 10 dans les applications de WB, IF, IHC, ELISA, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cytokeratin 10 Protéine recombinante Ag13136
• Nom complet: keratin 10
• Masse moléculaire calculée: 584 aa, 59 kDa
• Poids moléculaire observé: 50-59 kDa
• Numéro d’acquisition GenBank: BC034697
• Symbole du gène: Cytokeratin 10
• Identification du gène (NCBI): 3858
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a type I keratin, keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia.

Publications

Species	Application	Title
rat	IF	Cell Mol Biol Lett; The role of NPY2R/NFATc1/DYRK1A regulatory axis in sebaceous glands for sebum synthesis; Authors - Tao Yang
mouse	IF	Aging Cell; Nociceptive transient receptor potential canonical 7 (TRPC7) mediates aging-associated tumorigenesis induced by ultraviolet B.; Authors - Wen-Li Hsu
mouse	IHC	Life Sci; The potential of Diosgenin in treating psoriasis: Studies from HaCaT keratinocytes and imiquimod-induced murine model.; Authors - Shidi Wu
human	IF	mBio; Commensal-Related Changes in the Epidermal Barrier Function Lead to Alterations in the Benzo[a]Pyrene Metabolite Profile and Its Distribution in 3D Skin.; Authors - Lisa Lemoine
human	IF	Eur Surg Res; A novel combination of keratinocyte and autologous microskin grafting to repair full thickness skin loss; Authors - Kai Yin
mouse	IF	Int J Biol Macromol; Skin-adaptive film dressing with smart-release of growth factors accelerated diabetic wound healing; Authors - Yingzheng Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joshua (Verified Customer) (07-27-2019)

Cells differentiated at air-liquid interface. Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Bright staining with minimal background.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human Keratinocytes

Cytokeratin 10 Antibody Immunofluorescence, validation (1:500 dilution) in Human Keratinocytes (Cat no:18343-1-AP)