Anticorps Polyclonal de lapin anti-Importin Beta

• Phare
• Validé par KD/KO

Importin Beta Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (1)

N° de cat : 10077-1-AP

Synonymes

KPNB1, Importin-90, Importin subunit beta-1, Importin subunit beta 1, Importin 90

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Galerie de données de validation

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  WB analysis using 10077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin beta antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin Beta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin Beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin Beta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10077-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin beta antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10077-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10077-1-AP (Importin beta antibody at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IHC staining of human colon cancer using 10077-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10077-1-AP (Importin beta antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 10077-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Importin Beta antibody (10077-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 10077-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Importin Beta antibody (10077-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 10077-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Importin Beta antibody (10077-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 10077-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human Importin Beta (10077-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A2780, cellules C6, cellules HeLa, cellules HT-1080, cellules NIH/3T3, cellules PC-12, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Informations sur le produit

10077-1-AP cible Importin Beta dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Importin Beta Protéine recombinante Ag0122
• Nom complet: karyopherin (importin) beta 1
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 90-97 kDa
• Numéro d’acquisition GenBank: BC003572
• Symbole du gène: KPNB1
• Identification du gène (NCBI): 3837
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Importin beta1, also known karyopherin beta or KPNB1, is a nuclear transport receptor that plays a key role in the nuclear import process. Importin beta1 and importin alpha work in concert to transport cargo into the nucleus, functioning as an essential component of the classical nuclear localization signal (NLS) pathway. Elevated expression of importin beta1 has been found in cervical tumors. (22125623)

Publications

Species	Application	Title
human	WB,IP	Cell Res; Neddylation of PTEN regulates its nuclear import and promotes tumor development.; Authors - Ping Xie
human	WB	Oncogene; KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells.; Authors - Zhi-Chuan Zhu ; KD Validated
human	WB,IP	J Transl Med; TC2N inhibits distant metastasis and stemness of breast cancer via blocking fatty acid synthesis; Authors - Xiang-Lin Hao
human	WB	Virol Sin; African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway.; Authors - Yisha Zhuo
human	WB	PLoS Pathog; Phospholipid scramblase 1 interacts with influenza A virus NP, impairing its nuclear import and thereby suppressing virus replication.; Authors - Weiyu Luo
human	WB	Biochem Biophys Res Commun; Nuclear import of Nkx2-2 is mediated by multiple pathways.; Authors - Wenbo Lin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

María (Verified Customer) (02-08-2022)

Works for WB (1:1000) and IF in permeabilized cells (1:100 ON). Validated with siRNA

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RPE-1/MSC