Anticorps Polyclonal de lapin anti-Kininogen 1

Kininogen 1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 11926-1-AP

Synonymes

BDK, Fitzgerald factor, HMWK, Ile Ser Bradykinin Bradykinin, Kallidin I Lysyl bradykinin, kininogen 1, KNG, KNG1

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Galerie de données de validation

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  WB analysis of HeLa using 11926-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11926-1-AP (Kininogen 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11926-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11926-1-AP (Kininogen 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11926-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11926-1-AP (Kininogen 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11926-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11926-1-AP (Kininogen 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 11926-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 11926-1-AP (Kininogen 1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11926-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11926-1-AP (Kininogen 1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 11926-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 11926-1-AP (Kininogen 1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 11926-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11926-1-AP (Kininogen 1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11926-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11926-1-AP (Kininogen 1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 11926-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11926-1-AP (Kininogen 1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 11926-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11926-1-AP (Kininogen 1 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human endometrial cancer using 11926-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 11926-1-AP (Kininogen 1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human endometrial cancer using 11926-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 11926-1-AP (Kininogen 1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 11926-1-AP

  Immunofluorescent analysis of HepG2 cells, using KNG1 antibody 11926-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A2780, cellules COLO 320, cellules MCF-7
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer de l'endomètre humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

11926-1-AP cible Kininogen 1 dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Kininogen 1 Protéine recombinante Ag2574
• Nom complet: kininogen 1
• Masse moléculaire calculée: 644 aa, 72 kDa
• Poids moléculaire observé: 60 kDa, 47 kDa
• Numéro d’acquisition GenBank: BC060039
• Symbole du gène: Kininogen 1
• Identification du gène (NCBI): 3827
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This gene is translated into High-molecular weight kininogen (HMWK) and low-molecular weight kininogen (LMWK) after alternative splicing. HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein. 11926-1-AP was generated against N-terminal 300 aa of HMW kininogen. It can bind both HMW and LMW kiniogen.

Publications

Species	Application	Title
human	WB,CoIP	EBioMedicine; An HBV susceptibility variant of KNG1 modulates the therapeutic effects of interferons α and λ1 in HBV infection by promoting MAVS lysosomal degradation; Authors - Bin Zhang
human	WB	Cell Physiol Biochem; Novel Serum Biomarkers Detected by Protein Array in Polycystic Ovary Syndrome with Low Progesterone Level.; Authors - Qin Zheng
rat	WB	PLoS One; Proteomic analysis of rat serum revealed the effects of chronic sleep deprivation on metabolic, cardiovascular and nervous system.; Authors - Bo Ma
rat	WB	Biol Pharm Bull; Proteomic analysis reveals the renoprotective effect of Tribulus terrestris against obesity-related glomerulopathy in rats.; Authors - Yue-Hua Jiang
mouse	WB	Med Sci Monit; Isobaric Tagging for Relative and Absolute Protein Quantification (iTRAQ)-Based Quantitative Proteomics Analysis of Differentially Expressed Proteins 1 Week After Spinal Cord Injury in a Rat Model.; Authors - Shen Liu
rat	WB,IF	JID Innov; Kininogen-Nitric Oxide Signaling at Nearby Nonexcited Acupoints after Long-Term Stimulation.; Authors - Ting Wang