Anticorps Polyclonal de lapin anti-KLRG1

KLRG1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 10974-1-AP

Synonymes

2F1, CLEC15A, KLRG1, MAFA, MAFA 2F1, MAFA L, MAFA LIKE, MAFA like receptor, MAFAL

---

Galerie de données de validation

• 

  WB analysis using 10974-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10974-1-AP (KLRG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human peripheral blood leukocyte using 10974-1-AP

  Human peripheral blood leukocytes were subjected to SDS PAGE followed by western blot with 10974-1-AP (KLRG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10974-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10974-1-AP (KLRG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10974-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10974-1-AP (KLRG1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10974-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10974-1-AP (KLRG1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules Raji, leucocyte de sang périphérique humain

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

10974-1-AP cible KLRG1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KLRG1 Protéine recombinante Ag1407
• Nom complet: killer cell lectin-like receptor subfamily G, member 1
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC012621
• Symbole du gène: KLRG1
• Identification du gène (NCBI): 10219
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KLRG1 (killer cell lectin-like receptor subfamily G, member 1) is a C-type lectin inhibitory receptor that contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain. KLRG1 is expressed by subsets of NK and T cells, existing both as a monomer and as a disulfide-linked homodimer (PMID: 10741410; 19604491). It is considered to be a cell differentiation marker for NK and T cells and is strongly induced by viral and other infections (PMID: 20373518). Through interactions with members of the cadherin family, KLRG1 plays an inhibitory role on NK- and T-cell function (PMID: 19009530; 20373518).

Publications

Species	Application	Title
human		Brain; Highly differentiated cytotoxic T cells in inclusion body myositis.; Authors - Steven A Greenberg
human	IF	J Neurol; Morphological and molecular comparison of HIV-associated and sporadic inclusion body myositis; Authors - Sinja Vogt