Anticorps Polyclonal de lapin anti-Kallikrein 5

Kallikrein 5 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 10514-2-AP

Synonymes

Kallikrein 5, Kallikrein like protein 2, kallikrein related peptidase 5, KLK L2, KLK5, KLKL2, SCTE, Stratum corneum tryptic enzyme

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Galerie de données de validation

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  IHC staining of human skin using 10514-2-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10514-2-AP (Kallikrein 5 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 10514-2-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10514-2-AP (Kallikrein 5 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse skin using 10514-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10514-2-AP (Kallikrein 5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 10514-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10514-2-AP (Kallikrein 5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10514-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10514-2-AP (Kallikrein 5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10514-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10514-2-AP (Kallikrein 5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IHC: tissu cutané humain, tissu cutané de souris, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

10514-2-AP cible Kallikrein 5 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Kallikrein 5 Protéine recombinante Ag0799
• Nom complet: kallikrein-related peptidase 5
• Masse moléculaire calculée: 32 kDa
• Numéro d’acquisition GenBank: BC008036
• Symbole du gène: KLK5
• Identification du gène (NCBI): 25818
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KLK5(Kallikrein-5) is also named as SCTE and belongs to the kallikrein subfamily. This protein is predicted to be a secreted serine protease, and the enzyme is found to have proteolytic activity. In serum and ascites fluid, the protein is present in two forms, one at a relatively lower molecular mass (around 50 kDa), and another one around 150-180 kDa and native KLK5 is highly glycosylated or that it may interact with the gel filtration column, leading to delayed retention(PMID:12873991). The activation of the enzyme has been shown to require cleavage of an arginine residue (Arg66-Ile67), suggesting that a trypsin-like serine protease may be involved in this process(PMID:15713679).

Publications

Species	Application	Title
human	IHC	Cancer Biomark; Salivary KLK5 and uPA are potential biomarkers for malignant transformation of OLK and OLP; Authors - Yingzhu Kang
human	WB,IF	J Dermatol Sci; Dichotomous effect of ultraviolet B on the expression of corneodesmosomal enzymes in human epidermal keratinocytes.; Authors - Nin Megumi M
human	WB,IHC	Cell Death Dis; Long noncoding RNA HEIH depletion depresses esophageal carcinoma cell progression by upregulating microRNA-185 and downregulating KLK5.; Authors - Bing Wang