Anticorps Polyclonal de lapin anti-KLF12

• Phare
• Validé par KD/KO

KLF12 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ChIP, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 13156-1-AP

Synonymes

AP 2rep, AP2REP, HSPC122, KLF12, Krueppel like factor 12, Kruppel like factor 12

---

Galerie de données de validation

• 

  WB analysis using 13156-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13156-1-AP (KLF12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 13156-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13156-1-AP (KLF12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Raji, tissu rénal humain

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

13156-1-AP cible KLF12 dans les applications de WB, IHC, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KLF12 Protéine recombinante Ag3788
• Nom complet: Kruppel-like factor 12
• Masse moléculaire calculée: 402 aa, 44 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC019680
• Symbole du gène: KLF12
• Identification du gène (NCBI): 11278
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KLF12, also named as AP2REP or HSPC122, is a 402 amino acid protein, which contains three C2H2-type zinc fingers and belongs to the Sp1 C2H2-type zinc-finger protein family. KLF12 localizes in the nucleus and confers strong transcriptional repression to the AP-2-alpha gene. KLF12 is atranscriptional silencer to the AP2-alpha gene, mediating positive and negative regulation of the AP2-A expression trhough a set of overlapping cis-regulatory promoters elements.

Publications

Species	Application	Title
human	WB	Oncogene; Tumour-suppression function of KLF12 through regulation of anoikis.; Authors - N Godin-Heymann ; KD Validated
human	WB	Front Oncol; circ_NOTCH3 Functions as a Protooncogene Competing With miR-205-5p, Modulating KLF12 Expression and Promoting the Development and Progression of Basal-Like Breast Carcinoma.; Authors - Bing Guan
mouse	WB	J Ethnopharmacol; Salvia miltiorrhiza bunge exerts anti-oxidative effects through inhibiting KLF10 expression in vascular smooth muscle cells exposed to high glucose.; Authors - Jing Zhou
human	WB	Onco Targets Ther; The role of miR-382-5p in glioma cell proliferation, migration and invasion.; Authors - Jinjin Wang
human	WB,IHC	Biochem Biophys Res Commun; Krüppel-like factor 12 negatively regulates human endometrial stromal cell decidualization.; Authors - Shen Xiaoyue X
mouse	WB	Genes Dis; All-trans retinoic acid reverses malignant biological behavior of hepatocarcinoma cells by regulating miR-200 family members.; Authors - Jiejie Cui