Anticorps Polyclonal de lapin anti-KIF5A

• Phare
• Validé par KD/KO

KIF5A Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (1)

N° de cat : 21186-1-AP

Synonymes

D12S1889, KIF5A, kinesin family member 5A, Kinesin heavy chain isoform 5A, MY050, Neuronal kinesin heavy chain, NKHC, NKHC1, SPG10

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Galerie de données de validation

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  WB analysis using 21186-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21186-1-AP (KIF5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 21186-1-AP

  IP result of anti-KIF5A (IP:21186-1-AP, 4ug; Detection:21186-1-AP 1:2000) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 21186-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21186-1-AP (KIF5A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21186-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21186-1-AP (KIF5A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 21186-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21186-1-AP (KIF5A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 21186-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21186-1-AP (KIF5A antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of PC-12 using 21186-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using KIF5A antibody (21186-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules PC-12,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

21186-1-AP cible KIF5A dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KIF5A Protéine recombinante Ag15518
• Nom complet: kinesin family member 5A
• Masse moléculaire calculée: 1032 aa, 117 kDa
• Poids moléculaire observé: 118-120 kDa
• Numéro d’acquisition GenBank: BC146670
• Symbole du gène: KIF5A
• Identification du gène (NCBI): 3798
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Kinesin superfamily proteins (KIFs) are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. KIF5A is expressed exclusively in neurons and transports neuronal cargoes into axons and dendrites. KIF5A mutations have been associated with Charcot-Marie-Tooth Type 2, an axonal peripheral neuropathy characterized by progressive loss of peripheral sensation and muscle wasting.

Publications

Species	Application	Title
mouse	WB	Exp Neurol; Axonal injury mediated by neuronal p75NTR/TRAF6/JNK pathway contributes to cognitive impairment after repetitive mTBI; Authors - Gang Liu
human	WB	Hum Mol Genet; Mitochondrial transport mediates survival of retinal ganglion cells in affected LHON patients.; Authors - Tien-Chun Yang
rat	WB	Biol Sex Differ; Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features.; Authors - Marzia Pesaresi
mouse	WB	Alzheimers Res Ther; Normal levels of KIF5 but reduced KLC1 levels in both Alzheimer disease and Alzheimer disease in Down syndrome: evidence suggesting defects in anterograde transport.; Authors - Xu-Qiao Chen
mouse	WB	JCI Insight; Transcriptional control of a collagen deposition and adhesion process that promotes lung adenocarcinoma growth and metastasis.; Authors - Xiaochao Tan ; KD Validated
human	IHC	Front Oncol; KIF5A upregulation in hepatocellular carcinoma: A novel prognostic biomarker associated with unique tumor microenvironment status; Authors - Qi Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xuqiao (Verified Customer) (08-25-2020)

It specifically probes both endogenous and transfected KIF5A from either human or mouse.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T cell

KIF5A Antibody Western Blot validation (1:2000 dilution) in HEK293T cell (Cat no:21186-1-AP)