Anticorps Polyclonal de lapin anti-KIF3C

KIF3C Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 14333-1-AP

Synonymes

KIF3C, kinesin family member 3C, Kinesin like protein KIF3C

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Galerie de données de validation

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  WB analysis of Y79 using 14333-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 14333-1-AP (KIF3C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14333-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14333-1-AP (KIF3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14333-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14333-1-AP (KIF3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14333-1-AP

  IP result of anti-KIF3C (IP:14333-1-AP, 4ug; Detection:14333-1-AP 1:500) with mouse brain tissue lysate 3440ug.

• 

  IHC staining of human gliomas using 14333-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14333-1-AP (KIF3C antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 14333-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14333-1-AP (KIF3C antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 14333-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 14333-1-AP (KIF3C antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Y79, cellules HeLa, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de gliome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

14333-1-AP cible KIF3C dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KIF3C Protéine recombinante Ag5619
• Nom complet: kinesin family member 3C
• Masse moléculaire calculée: 89 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC042486
• Symbole du gène: KIF3C
• Identification du gène (NCBI): 3797
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Dev Cell; ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.; Authors - Jie Ran
mouse	WB	Elife; Loss of Tsc1 in cerebellar Purkinje cells induces transcriptional and translation changes in FMRP target transcripts; Authors - Jasbir Singh Dalal
human	IHC	Aging (Albany NY); METTL3-mediated m6A modification of KIF3C-mRNA promotes prostate cancer progression and is negatively regulated by miR-320d.; Authors - Honggui Ma
human	WB	Biomed Res Int; KIF3C Promotes Proliferation, Migration, and Invasion of Glioma Cells by Activating the PI3K/AKT Pathway and Inducing EMT.; Authors - Yang Gao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Abigail (Verified Customer) (07-10-2024)

We used this antibody for WB of mouse brain cortex homogenate and got clear bands around 100 kDa after a 1:1000 overnight incubation.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse brain cortex