Anticorps Polyclonal de lapin anti-KIF26B

• Phare
• Validé par KD/KO

KIF26B Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain et plus (2)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (1)

N° de cat : 17422-1-AP

Synonymes

KIF26B, kinesin family member 26B, Kinesin like protein KIF26B

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Galerie de données de validation

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  WB analysis of MDCK using 17422-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 17422-1-AP (KIF26B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17422-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17422-1-AP (KIF26B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17422-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17422-1-AP (KIF26B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 17422-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17422-1-AP (KIF26B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 17422-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17422-1-AP (KIF26B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 17422-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17422-1-AP (KIF26B Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 17422-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17422-1-AP (KIF26B Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules MDCK, cellules HEK-293, cellules MCF-7
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 8 publications below
• IHC: See 7 publications below
• IF: See 3 publications below

Informations sur le produit

17422-1-AP cible KIF26B dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons canin, Humain

• Réactivité: canin, Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KIF26B Protéine recombinante Ag11446
• Nom complet: kinesin family member 26B
• Masse moléculaire calculée: 2108aa,224 kDa; 162aa,17 kDa
• Poids moléculaire observé: 224-230 kDa, 17-22 kDa
• Numéro d’acquisition GenBank: BC042481
• Symbole du gène: KIF26B
• Identification du gène (NCBI): 55083
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KIF26B is a member of kinesin-11 family and plays important role in embryogenesis. It is a downstram target of Sall1 and is essential for kidney development. KIF26B is also involved in tumorigenesis.

Publications

Species	Application	Title
human,mouse	WB,IHC	Nat Commun; Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.; Authors - Shujie Chen ; KD Validated
human	IHC,WB	Biomed Pharmacother; KIF26B promotes cell proliferation and migration through the FGF2/ERK signaling pathway in breast cancer.; Authors - Yan Teng
human,mouse	WB,IF	J Bone Miner Res; KIF26B Silencing Prevents Osseous Transdifferentiation of Progenitor/Stem Cells and Attenuates Ectopic Calcification in a Murine Model.; Authors - Mingming Yan ; KD Validated
human	IF	J Ginseng Res; Ginsenoside Rh2 reduces m6A RNA methylation in cancer via the KIF26B-SRF positive feedback loop; Authors - Chunmei Hu
human	IHC	Cell Cycle; Noncoding RNAs-based high KIF26B expression correlates with poor prognosis and tumor immune infiltration in colon cancer; Authors - Zhihong Liu
mouse	IF	Biochem Biophys Res Commun; Novel mechanistic role of Kif26b in adipogenic differentiation of murine multipotent stromal cells.; Authors - Mingming Yan ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Abigail (Verified Customer) (06-17-2024)

We had a lot of issues with this antibody due to difficulties transferring the high molecular weight protein. When we were able to get a good transfer as seen on the Ponceau, it was pretty hit or miss on if the antibody worked. We only had one quantifiable blot, the rest were not able to be transferred due to poor signal.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Brain cortex