Anticorps Polyclonal de lapin anti-KIF20A

• Phare
• Validé par KD/KO

KIF20A Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

10

Avis (2)

N° de cat : 15911-1-AP

Synonymes

GG10_2, KIF20A, kinesin family member 20A, Kinesin like protein KIF20A, MKLP2, RAB6KIFL, Rabkinesin 6

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Galerie de données de validation

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  WB analysis using 15911-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 15911-1-AP (KIF20A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15911-1-AP

  WB result of KIF20A antibody (15911-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF20A transfected HeLa cells.

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  IP experiment of HEK-293 using 15911-1-AP

  IP result of anti-KIF20A (IP:15911-1-AP, 4ug; Detection:15911-1-AP 1:500) with HEK-293 cells lysate 1200ug.

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  IHC staining of human breast cancer using 15911-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15911-1-AP (KIF20A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15911-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15911-1-AP (KIF20A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15911-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15911-1-AP (KIF20A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HeLa
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

15911-1-AP cible KIF20A dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KIF20A Protéine recombinante Ag8700
• Nom complet: kinesin family member 20A
• Masse moléculaire calculée: 890 aa, 100 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC012999
• Symbole du gène: KIF20A
• Identification du gène (NCBI): 10112
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KIF20A, also known as RAB6KIFL or MKlp2, is a kinesin originally identified as a binding partner for the rab6 GTPase involved in protein transport at the Golgi apparatus. KIF20A belongs to a large family of motor proteins that accumulate in mitotic cells and is required for normal cell division. KIF20A is thought to be involved in carcinogenesis. In normal tissues KIF20A is almost undetectable while it is overexpressed in various cancers. KIF20A has been identified as a novel promising candidate for anticancer immunotherapy.

Publications

Species	Application	Title
human	WB,IF	Nat Commun; Coupling fission and exit of RAB6 vesicles at Golgi hotspots through kinesin-myosin interactions.; Authors - Stéphanie Miserey-Lenkei
human	WB	Proteomics; Genistein-induced mitotic arrest of gastric cancer cells by downregulating KIF20A, a proteomics study.; Authors - Yan Guang-Rong GR
human	IHC	J Cancer; Five-gene signature associating with Gleason score serve as novel biomarkers for identifying early recurring events and contributing to early diagnosis for Prostate Adenocarcinoma.; Authors - Lingyu Zhang
		J Biochem Mol Toxicol; Bisphenol A triggers the malignancy of acute myeloid leukemia cells via regulation of IL-4 and IL-6.; Authors - Suwei Zhang
human	WB	Dis Markers; KIF20A Predicts Poor Survival of Patients and Promotes Colorectal Cancer Tumor Progression through the JAK/STAT3 Signaling Pathway.; Authors - Qi Zhang
human	IF	Science; The mechanism of acentrosomal spindle assembly in human oocytes; Authors - Tianyu Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (04-03-2024)

Not suitable for IF at all. showed many blobs staining all over the field, and cannot label any Golgi staining.

• Applications: Immunofluorescence

FH

Juliane (Verified Customer) (10-25-2022)

Golgi and nucleus staining ok but cytoplasmic difficult

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa

KIF20A Antibody Immunofluorescence validation (1:500 dilution) in HeLa (Cat no:15911-1-AP)