Anticorps Polyclonal de lapin anti-KIF18A

• Phare
• Validé par KD/KO

KIF18A Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 19245-1-AP

Synonymes

KIF18A, kinesin family member 18A, Kinesin like protein KIF18A, Marrow stromal KIF18A, MS KIF18A

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Galerie de données de validation

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  WB analysis of Jurkat using 19245-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19245-1-AP (KIF18A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19245-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19245-1-AP (KIF18A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 19245-1-AP

  IP result of anti-KIF18A (IP:19245-1-AP, 4ug; Detection:19245-1-AP 1:500) with Jurkat cells lysate 2400ug.

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  IHC staining of human colon cancer using 19245-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19245-1-AP (KIF18A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 19245-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19245-1-AP (KIF18A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 19245-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19245-1-AP (KIF18A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 19245-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19245-1-AP (KIF18A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 19245-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19245-1-AP (KIF18A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 19245-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19245-1-AP (KIF18A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du sein humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 8 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

19245-1-AP cible KIF18A dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KIF18A Protéine recombinante Ag6166
• Nom complet: kinesin family member 18A
• Masse moléculaire calculée: 102 kDa
• Poids moléculaire observé: 102 kDa
• Numéro d’acquisition GenBank: BC048347
• Symbole du gène: KIF18A
• Identification du gène (NCBI): 81930
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KIF18A belongs to the Kinesin family. KIF18A binds to microtubules and moves along them using energy from ATP hydrolysis. KIF18A is related to the localization of membrane organelles and protein complexes, as well as the maintenance of microtubule dynamics and mitosis. KIF18A expression is low in normal tissues and high in solid tumors, including breast cancer, hepatocellular carcinoma and lung adenocarcinoma, colorectal cancer, renal cell carcinoma, and head and neck squamous cell carcinoma. The molecular weight of KIF18A is 102 kDa (PMID: 36830695).

Publications

Species	Application	Title
human	IHC	Oncotarget; High KIF18A expression correlates with unfavorable prognosis in primary hepatocellular carcinoma.; Authors - Weijia Liao
human	WB	Oncotarget; Exclusive destruction of mitotic spindles in human cancer cells.; Authors - Leonid Visochek
mouse	IF	Sci Rep; PMID: 29229923; Authors - Sara Bizzotto
human	WB,IHC	Front Genet; Prognostic Biomarker KIF18A and Its Correlations With Immune Infiltrates and Mitosis in Glioma.; Authors - Bing-Yan Tao
human	WB	Dis Markers; Kinesin Family Member 18A (KIF18A) Contributes to the Proliferation, Migration, and Invasion of Lung Adenocarcinoma Cells In Vitro and In Vivo.; Authors - Fu-Tao Chen ; KD Validated
mouse	WB,IF	Cell Div; Kif18a regulates Sirt2-mediated tubulin acetylation for spindle organization during mouse oocyte meiosis.; Authors - Feng Tang ; KD Validated