Anticorps Polyclonal de lapin anti-KIAA0513

KIAA0513 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 21478-1-AP

Synonymes

KIAA0513

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Galerie de données de validation

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  WB analysis of mouse brain using 21478-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21478-1-AP (KIAA0513 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 21478-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21478-1-AP (KIAA0513 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 21478-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21478-1-AP (KIAA0513 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 21478-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21478-1-AP (KIAA0513 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 21478-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21478-1-AP (KIAA0513 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 21478-1-AP

  Immunofluorescent analysis of HepG2 cells, using KIAA0513 antibody 21478-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu de cervelet de souris, tissu rénal de souris, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

21478-1-AP cible KIAA0513 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KIAA0513 Protéine recombinante Ag15624
• Nom complet: KIAA0513
• Masse moléculaire calculée: 411 aa, 47 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC030280
• Symbole du gène: KIAA0513
• Identification du gène (NCBI): 9764
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KIAA0513 was identified as upregulated in subjects with schizophrenia. The mRNA of KIAA0513 is ubiquitously expressed but is enriched in the brain, particularly in the cerebellum. The interacting protein analysis indicated that KIAA0513 is involved in both synaptic and apoptotic signaling. (PMID: 17010949) Several isoforms of KIAA0513 (47/46/34 kDa) exist due to the alternative splicing. This antibody is expected to recognize all of isoforms of KIAA0513.