Anticorps Polyclonal de lapin anti-KDM4A

KDM4A Polyclonal Antibody for WB, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 29977-1-AP

Synonymes

JMJD2, JmjC domain-containing histone demethylation protein 3A, JHDM3A, EC:1.14.11.66, [histone H3]-trimethyl-L-lysine(9) demethylase 4A

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Galerie de données de validation

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  WB analysis using 29977-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29977-1-AP (KDM4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 29977-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 29977-1-AP (KDM4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 29977-1-AP

  IP result of anti-KDM4A (IP:29977-1-AP, 4ug; Detection:29977-1-AP 1:1500) with MCF-7 cells lysate 2120 ug.

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  IP experiment of A549 using 29977-1-AP

  IP result of anti-KDM4A (IP:29977-1-AP, 4ug; Detection:29977-1-AP 1:1500) with A549 cells lysate 800 ug.

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  FC experiment of A549 using 29977-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human KDM4A (29977-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules MCF-7, cellules MDA-MB-231, cellules NCL-H1299, tissu pulmonaire de souris
• Résultats positifs en IP: cellules MCF-7, cellules A549
• Résultats positifs en FC (Intra): cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

29977-1-AP cible KDM4A dans les applications de WB, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KDM4A Protéine recombinante Ag31992
• Nom complet: lysine (K)-specific demethylase 4A
• Masse moléculaire calculée: 1064 aa, 121 kDa
• Poids moléculaire observé: 130-150 kDa
• Numéro d’acquisition GenBank: BC002558
• Symbole du gène: KDM4A
• Identification du gène (NCBI): 9682
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KDM4A also named as JHDM3A, JMJD2, or KIAA0677 is a 1066 amino acid protein, which contains one JmjC domain, two Tudor domains and two PHD-type zinc fingers. KDM4A localizes in nucleus and belongs to the JHDM3 histone demethylase family. KDM4A is histone de-methylase that specifically demethylates Lys 9 and Lys 36 residues of Histone H3, thereby playing a central role in histone code. JMJD2A demethylation of lysine residues will generate formaldehyde and succinate. KDM4A also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. JMJD2A is a ubiquitously expressed nuclear protein. KDM4A exists as two isoforms. The short isoform of KDM4A in which the N-terminal demethylase domains has been deleted. The molecular weight of short isoform is 55Da.

Publications

Species	Application	Title
human	WB	Cell Commun Signal; HPV11 targeting KDM4A regulates the polarization of macrophage M1 and promotes the development of nasal inverted papilloma; Authors - Liying Zheng ; KO Validated

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