Anticorps Polyclonal de lapin anti-KDM4A

• Phare
• Validé par KD/KO

KDM4A Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, chIP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 24943-1-AP

Synonymes

JHDM3A, JMJD2, JMJD2A, KDM4A, KIAA0677, lysine (K) demethylase 4A, Lysine specific demethylase 4A

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 24943-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24943-1-AP (KDM4A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 24943-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24943-1-AP (KDM4A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

24943-1-AP cible KDM4A dans les applications de WB, chIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KDM4A Protéine recombinante Ag20747
• Nom complet: lysine (K)-specific demethylase 4A
• Masse moléculaire calculée: 1064 aa, 121 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC002558
• Symbole du gène: KDM4A
• Identification du gène (NCBI): 9682
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KDM4A also named as JHDM3A, JMJD2, or KIAA0677 is a 1066 amino acid protein, which contains one JmjC domain, two Tudor domains and two PHD-type zinc fingers. KDM4A localizes in nucleus and belongs to the JHDM3 histone demethylase family. KDM4A is histone de-methylase that specifically demethylates Lys 9 and Lys 36 residues of Histone H3, thereby playing a central role in histone code. JMJD2A demethylation of lysine residues will generate formaldehyde and succinate. KDM4A also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. JMJD2A is a ubiquitously expressed nuclear protein. KDM4A exists as two isoforms. The short isoform of KDM4A in which the N-terminal demethylase domains has been deleted. The molecular weight of short isoform is 55Da.

Publications

Species	Application	Title
mouse	WB	Front Pharmacol; Folic Acid Attenuates Glial Activation in Neonatal Mice and Improves Adult Mood Disorders Through Epigenetic Regulation.; Authors - Tiantian Zhao
human	WB,chIP	Biochem Biophys Res Commun; The histone demethylase Kdm4 suppresses activation of hepatic stellate cell by inducing MiR-29 transcription.; Authors - Ming Kong ; KD Validated