Anticorps Polyclonal de lapin anti-Kir2.1

Kir2.1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 19965-1-AP

Synonymes

HHBIRK1, HHIRK1, hIRK1, IRK 1, IRK1, KCNJ2, Kir2.1, LQT7, SQT3

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Galerie de données de validation

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  WB analysis of A549 using 19965-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 19965-1-AP (Kir2.1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 19965-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 19965-1-AP (Kir2.1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 19965-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19965-1-AP (Kir2.1 antibody at dilution of 1:50.

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  IHC staining of human brain using 19965-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19965-1-AP (Kir2.1 antibody at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: cellules A549
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 2 publications below

Informations sur le produit

19965-1-AP cible Kir2.1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, Lapin, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: potassium inwardly-rectifying channel, subfamily J, member 2
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 50 kDa, 60 kDa
• Numéro d’acquisition GenBank: NM_000891
• Symbole du gène: Kir2.1
• Identification du gène (NCBI): 3759
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KCNJ2, also named as HHBIRK1, HHIRK1, IRK1, KIR2.1, LQT7 and SQT3, belongs to the inward rectifier-type potassium channel family. KCNJ2 probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ2 can be blocked by extracellular barium or cesium. Defects in KCNJ2 are the cause of long QT syndrome type 7 (LQT7). Defects in KCNJ2 are the cause of short QT syndrome type 3 (SQT3). The antibody recognizes the C-term of KCNJ2.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Kir2.1-mediated membrane potential promotes nutrient acquisition and inflammation through regulation of nutrient transporters.; Authors - Weiwei Yu
human	WB	Stem Cell Res Ther; Overexpression of KCNJ2 enhances maturation of human-induced pluripotent stem cell-derived cardiomyocytes; Authors - Jingjun Zhou
rabbit	WB	J Mol Cell Cardiol; Altered long non-coding RNA expression profile in rabbit atria with atrial fibrillation: TCONS_00075467 modulates atrial electrical remodeling by sponging miR-328 to regulate CACNA1C.; Authors - Zhan Li
rabbit	WB	J Cell Mol Med; Long noncoding RNA TCONS-00106987 promotes atrial electrical remodelling during atrial fibrillation by sponging miR-26 to regulate KCNJ2.; Authors - Juanjuan Du
mouse	IHC	Funct Integr Genomics; Knockdown of circZMIZ1 enhances the anti-tumor activity of CD8+ T cells to alleviate hepatocellular carcinoma; Authors - Xi Li
mouse	WB	Hypertens Res; Administration of USP7 inhibitor p22077 alleviates Angiotensin II (Ang II)-induced atrial fibrillation in Mice; Authors - Yu Wang