Anticorps Polyclonal de lapin anti-KCNJ15

• Phare
• Validé par KD/KO

KCNJ15 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 15988-1-AP

Synonymes

IRKK, KCNJ14, KCNJ15, KIR1.3, KIR4.2

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Galerie de données de validation

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  WB analysis of HeLa using 15988-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15988-1-AP (KCNJ15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15988-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15988-1-AP (KCNJ15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

15988-1-AP cible KCNJ15 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: KCNJ15 Protéine recombinante Ag8795
• Nom complet: potassium inwardly-rectifying channel, subfamily J, member 15
• Masse moléculaire calculée: 375 aa, 43 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC013327
• Symbole du gène: KCNJ15
• Identification du gène (NCBI): 3772
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KCNJ15, also named as KIR1.3, KIR4.2, and IRKK, is a multi-pass membrane protein belonging to the inwardly-rectifying potassium channel (KIR) family. KIR channels perform functions as diverse as the regulation of resting membrane potential, maintenance of potassium ions homeostasis, control of heart rate and hormone secretion. Cloned from human kidney, the KCNJ15 gene is localized on chromosome 21 in the Down syndrome chromosome region 1 (DCR1) and has been identified as a type 2 diabetes-associated risk gene. The gene is most readily expressed in the pancreas and kidney and less in the lung. (PMID: 8995301; 9299242; 20085713)

Publications

Species	Application	Title
human	WB, IF	Nat Commun; KCNJ15/Kir4.2 couples with polyamines to sense weak extracellular electric fields in galvanotaxis.; Authors - Ken-Ichi Nakajima ; KD Validated
mouse	IHC	DNA Res; Identification of novel genes selectively expressed in the follicle-associated epithelium from the meta-analysis of transcriptomics data from multiple mouse cell and tissue populations.; Authors - Kobayashi Atsushi A
human	IHC	Ann Surg Oncol; KCNJ15 Expression and Malignant Behavior of Esophageal Squamous Cell Carcinoma.; Authors - Shunsuke Nakamura