Anticorps Polyclonal de lapin anti-Gamma Catenin

Gamma Catenin Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 11146-1-AP

Synonymes

γ-Catenin, ARVD12, Catenin gamma, CTNNG, Desmoplakin 3, Desmoplakin III, DP3, DPIII, junction plakoglobin, JUP, gamma Catenin, PDGB, PKGB

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Galerie de données de validation

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  WB analysis of mouse heart using 11146-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11146-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11146-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11146-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T-47D using 11146-1-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HT-29 using 11146-1-AP

  IP result of anti-Gamma Catenin (IP:11146-1-AP, 4ug; Detection:11146-1-AP 1:1000) with HT-29 cells lysate 1040 ug.

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  IHC staining of human pancreas cancer using 11146-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11146-1-AP (Plakoglobin/gamma catenin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 11146-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50.

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  IHC staining of human colon cancer using 11146-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50.

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  IHC staining of human skin using 11146-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50.

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  IHC staining of human skin using 11146-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50.

• 

  IHC staining of human breast cancer using 11146-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50.

• 

  IHC staining of human breast cancer using 11146-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules A431, cellules HeLa, cellules T-47D, tissu cardiaque humain
• Résultats positifs en IP: cellules HT-29,
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu cutané humain, tissu de cancer du côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

11146-1-AP cible Gamma Catenin dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Gamma Catenin Protéine recombinante Ag1627
• Nom complet: junction plakoglobin
• Masse moléculaire calculée: 82 kDa
• Poids moléculaire observé: 82 kDa
• Numéro d’acquisition GenBank: BC011865
• Symbole du gène: Gamma Catenin
• Identification du gène (NCBI): 3728
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Plakoglobin (γ-catenin), a member of the Armadillo proteins, plays important roles in cell adhesion with α-catenin and β-catenin, is involved in Wnt signaling, and is translocated into the nucleus and binds LEF1. Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. Plakoglobin links cadherins to the actin cytoskeleton. Mutations in plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.

Publications

Species	Application	Title
human	WB	J Clin Invest; The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer.; Authors - Wanjin Li
mouse	WB	Oncogene; Loss of giant obscurins from breast epithelium promotes epithelial-to-mesenchymal transition, tumorigenicity and metastasis.; Authors - M Shriver
human	WB	PLoS One; Quantitative Proteomic Profiling the Molecular Signatures of Annexin A5 in Lung Squamous Carcinoma Cells.; Authors - Bing Sun
mouse	WB	Biochem Biophys Res Commun; Plakoglobin is involved in cytoskeletal rearrangement of podocytes under the regulation of UCH-L1.; Authors - Yili Fang
mouse,rat	WB,IF,IP	Cell Rep; CYLD deubiquitinates plakoglobin to promote Cx43 membrane targeting and gap junction assembly in the heart; Authors - Wei Xie
human	IHC	Int J Mol Sci; Diagnostic and Prognostic Markers for Pancreatitis and Pancreatic Ductal Adenocarcinoma; Authors - Havish S Kantheti