Anticorps Polyclonal de lapin anti-JUNB

• Phare
• Validé par KD/KO

JUNB Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 10486-1-AP

Synonymes

AP 1, jun B proto oncogene, JUNB, Transcription factor jun B

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Galerie de données de validation

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  WB analysis of MCF-7 using 10486-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10486-1-AP (JUNB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 10486-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 10486-1-AP (JUNB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10486-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10486-1-AP (JUNB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10486-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10486-1-AP (JUNB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 10486-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10486-1-AP (JUNB antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10486-1-AP

  IP result of anti-JUNB (IP:10486-1-AP, 4ug; Detection:10486-1-AP 1:500) with HeLa cells lysate 1200ug.

• 

  IHC staining of human gliomas using 10486-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10486-1-AP (JUNB antibody) at dilution of 1:50 (under 25x lens).

• 

  IHC staining of human ovary using 10486-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 10486-1-AP (JUNB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 10486-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 10486-1-AP (JUNB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 10486-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10486-1-AP (JUNB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 10486-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10486-1-AP (JUNB antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of MCF-7 using 10486-1-AP

  Immunofluorescent analysis of MCF-7 cells using 10486-1-AP (JUNB antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10486-1-AP

  Immunofluorescent analysis of HeLa cells using 10486-1-AP (JUNB antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF7, cellules C6, cellules HeLa, cellules HT-1080
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de gliome humain, tissu ovarien humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

10486-1-AP cible JUNB dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: JUNB Protéine recombinante Ag0752
• Nom complet: jun B proto-oncogene
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 42 kDa, 43 kDa
• Numéro d’acquisition GenBank: BC004250
• Symbole du gène: JUNB
• Identification du gène (NCBI): 3726
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

JunB is one of the components of the Activator Protein-1 (AP-1) transcription complex that have been implicated in the control ofthe G0/G1 transtion in fibroblasts. AP-1 is a collection of dimers formed by members of the Jun-, Fos-, ATF- and Maf multigene families that bind to specific DNA regulatory elements called AP-1/12-O-tetradecanoylphorbol-13-acetate-responsive elements (TREs) and cAMP-responsive elements (CREs). JunB binds to the DNA sequence 5'-TGA[CG]TCA-3', and involves in the regulation of gene activity following the primary growth factor response. It also acts either as a tumor suppressor or as an oncogene depending on the cell and physiopathological context

Publications

Species	Application	Title
mouse	WB	Signal Transduct Target Ther; MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration; Authors - Jinpeng Du
mouse	WB	J Exp Med; USP38 critically promotes asthmatic pathogenesis by stabilizing JunB protein.; Authors - Siyuan Chen
mouse,rat	WB	Pharmacol Res; Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways.; Authors - Jiale Cai
human	WB	Am J Cancer Res; PMID: 31285951; Authors - Wen-Si Zhao
human	WB	J Proteome Res; Systematic Survey of the Regulatory Networks of the Long Noncoding RNA BANCR in Cervical Cancer Cells.; Authors - Bing Wang
human	WB	Exp Cell Res; An innovative immunotherapeutic strategy for rheumatoid arthritis: Selectively suppressing angiogenesis and osteoclast differentiation by fully human antibody targeting thymocyte antigen-1; Authors - Xuanxuan Hu